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Literature summary for 2.7.4.6 extracted from

  • Tokunaga, H.; Ishibashi, M.; Arisaka, F.; Arai, S.; Kuroki, R.; Arakawa, T.; Tokunaga, M.
    Residue 134 determines the dimer-tetramer assembly of nucleoside diphosphate kinase from moderately halophilic bacteria (2008), FEBS Lett., 582, 1049-1054.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ndk, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Pseudomonas sp.
gene ndk, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Halomonas sp. #593

Protein Variants

Protein Variants Comment Organism
A134E site-directed mutagenesis, the mutant forms a dimer in analogy to the wild-type enzyme of Halomonas sp., which possesses an E134 residue, overview Pseudomonas sp.
E134A site-directed mutagenesis, the mutant forms a tetramer in analogy to the wild-type enzyme of Pseudomonas sp., which possesses an A134 residue, overview Halomonas sp. #593

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Pseudomonas sp.
Mg2+
-
Halomonas sp. #593

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15592
-
2 * 15592 Halomonas sp. #593

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + TDP Pseudomonas sp.
-
ADP + TTP
-
r
ATP + TDP Halomonas sp. #593
-
ADP + TTP
-
r

Organism

Organism UniProt Comment Textmining
Halomonas sp. #593 Q83WH5 gene ndk
-
Pseudomonas sp.
-
gene ndk
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + TDP
-
Pseudomonas sp. ADP + TTP
-
r
ATP + TDP
-
Halomonas sp. #593 ADP + TTP
-
r

Subunits

Subunits Comment Organism
dimer 2 * 15592 Halomonas sp. #593
More structure modeling of wild-type and mutant enzyme, overview Pseudomonas sp.
More structure modeling of wild-type and mutant enzyme, overview Halomonas sp. #593
tetramer
-
Pseudomonas sp.

Synonyms

Synonyms Comment Organism
NDK
-
Pseudomonas sp.
NDK
-
Halomonas sp. #593
nucleoside diphosphate kinase
-
Pseudomonas sp.
nucleoside diphosphate kinase
-
Halomonas sp. #593

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas sp.
30
-
assay at Halomonas sp. #593

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pseudomonas sp.
7.5
-
assay at Halomonas sp. #593