Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli DH5alpha | Pisum sativum |
Protein Variants | Comment | Organism |
---|---|---|
H117A | activity almost completely abolished | Pisum sativum |
H117D/S119A | activity almost completely abolished | Pisum sativum |
S119A | reduced serine phosphorylation, only modest decrease of activity | Pisum sativum |
S69A | 6% and 14% of wild type activity using dCDP or dTDP as substrate respectively, changes in subunit composition with increasing number of tetramers and dimers | Pisum sativum |
S69A/S119A | activity almost completely abolished | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
dTDP | S69A mutant enzyme, 22°C | Pisum sativum | |
0.08 | - |
dTDP | S119A mutant enzyme, 22°C | Pisum sativum | |
0.09 | - |
dCDP | S69A mutant enzyme, 22°C | Pisum sativum | |
0.1 | - |
dTDP | wild type enzyme, 22°C | Pisum sativum | |
0.2 | - |
dCDP | wild type enzyme, 22°C | Pisum sativum | |
0.28 | - |
dCDP | S119A mutant enzyme, 22°C | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Pisum sativum | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent cation required for catalysis, Mg2+ preferred over other ions | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NDP | Pisum sativum | contributes to the maintenance of the cellular pools of all nucleosides triphosphates, broad substrate specificity, in plants involved in different signaling pathways | ADP + NTP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | Q9SP13 | L. cv Oregon sugarpod | - |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Pisum sativum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50 | - |
H117A mutant enzyme with dCDP as substrate, 22°C | Pisum sativum |
60 | - |
S69A/S119A mutant enzyme with dCDP as substrate, 22°C | Pisum sativum |
70 | - |
H117D/S119A mutant enzyme with dCDP as substrate, 22°C | Pisum sativum |
160 | - |
S69A mutant enzyme with dCDP as substrate, 22°C | Pisum sativum |
700 | - |
S69A mutant enzyme with dTDP as substrate, 22°C | Pisum sativum |
2400 | - |
S119A mutant enzyme with dCDP as substrate, 22°C | Pisum sativum |
2700 | - |
wild type enzyme with dCDP as substrate, 22°C | Pisum sativum |
3900 | - |
S119A mutant enzyme with dTDP as substrate, 22°C | Pisum sativum |
5100 | - |
wild type enzyme with dTDP as substrate, 22°C | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dCDP | - |
Pisum sativum | ADP + dCTP | - |
? | |
ATP + dTDP | - |
Pisum sativum | ADP + dTTP | - |
? | |
ATP + NDP | - |
Pisum sativum | ADP + NTP | - |
? | |
ATP + NDP | contributes to the maintenance of the cellular pools of all nucleosides triphosphates, broad substrate specificity, in plants involved in different signaling pathways | Pisum sativum | ADP + NTP | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | monomers are arranged as trimers of dimers, crystal structure analysis | Pisum sativum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
dCDP | S69A mutant enzyme, 22°C | Pisum sativum | |
17 | - |
dTDP | S69A mutant enzyme, 22°C | Pisum sativum | |
105 | - |
dCDP | S119A mutant enzyme, 22°C | Pisum sativum | |
106 | - |
dCDP | wild type enzyme, 22°C | Pisum sativum | |
120 | - |
dTDP | S119A mutant enzyme, 22°C | Pisum sativum | |
147 | - |
dTDP | wild type enzyme, 22°C | Pisum sativum |