Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.4.6 extracted from

  • Chopra, P.; Singh, A.; Koul, A.; Ramachandran, S.; Drlica, K.; Tyagi, A.K.; Singh, Y.
    Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis (2003), Eur. J. Biochem., 270, 625-634.
    View publication on PubMed
Search for more literature for 2.7.4.6

Cloned(Commentary)

Cloned (Comment) Organism
native and mutant enzymes expressed as His-tag fusion protein in Escherichia coli SG13009 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
H117Q significant loss of activity with ATP and GTP Mycobacterium tuberculosis
H49Q no effect on activity Mycobacterium tuberculosis
H53Q no effect on activity Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular enzyme is secreted to the culture medium Mycobacterium tuberculosis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14400
-
 x * 14400, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + NDP Mycobacterium tuberculosis contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages ADP + NTP
-
 ?
ATP + NDP Mycobacterium tuberculosis H37Rv contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages ADP + NTP
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzymes using His-tag Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NDP
-
 Mycobacterium tuberculosis ADP + NTP
-
 ?
ATP + NDP contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages Mycobacterium tuberculosis ADP + NTP
-
 ?
ATP + NDP
-
 Mycobacterium tuberculosis H37Rv ADP + NTP
-
 ?
ATP + NDP contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages Mycobacterium tuberculosis H37Rv ADP + NTP
-
 ?
GTP + NDP
-
 Mycobacterium tuberculosis GDP + NTP
-
 ?
GTP + NDP
-
 Mycobacterium tuberculosis H37Rv GDP + NTP
-
 ?

Subunits

Subunits Comment Organism
? x * 14400, SDS-PAGE Mycobacterium tuberculosis