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Literature summary for 2.7.4.6 extracted from

  • Agarwal, R.P.; Robison, B.; Parks, R.E.
    Nucleoside diphosphokinase from human erythrocytes (1978), Methods Enzymol., 51, 376-386.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate pI 7.3-isoform, DTT restores, but not in the presence of urea, ATP or dTDP protects Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-values of different pI-enzyme variants of ATP or GTP and TDP or CDP as substrates Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement, about half as effective as Mg2+, Mn2+ or Co2+, pI 7.3-enzyme variant Homo sapiens
Co2+ requirement Homo sapiens
Co2+ equally effective as Mg2+ or Mn2+, pI 7.3-enzyme variant Homo sapiens
Mg2+ equally effective as Mn2+ or Co2+, pI 7.3-enzyme variant Homo sapiens
Mn2+ requirement Homo sapiens
Mn2+ equally effective as Co2+, pI 7.3-enzyme variant Homo sapiens
Mn2+ equally effective as Mg2+ Homo sapiens
additional information no activation by Cu2+ or monovalent cations Homo sapiens
Zn2+ not Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000 100000 erythrocytic enzyme variants Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NDP + NTP Homo sapiens
-
NTP + NDP
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
marked isoelectric variability with pIs from 5.4 to 8.3 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate the catalytic mechanism involves a phosphoenzyme intermediate Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + CDP
-
Homo sapiens ADP + CTP
-
?
ATP + dTDP
-
Homo sapiens ADP + dTTP
-
?
GTP + CDP
-
Homo sapiens GDP + CTP
-
?
GTP + dTDP
-
Homo sapiens GDP + dTTP
-
?
NDP + NTP
-
Homo sapiens NTP + NDP
-
r
NTP + nucleoside diphosphate substrate: 5-fluoro-UTP Homo sapiens NDP + NTP
-
r
NTP + nucleoside diphosphate substrate 8-aza-UDP, human erythrocytic pI-variant 5.8 Homo sapiens NDP + NTP
-
r