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Literature summary for 2.7.4.6 extracted from

  • Jong, A.Y.; Ma, J.J.
    Saccharomyces cerevisiae nucleoside-diphosphate kinase: purification, characterization, and substrate specificity (1991), Arch. Biochem. Biophys., 291, 241-246.
    View publication on PubMed

General Stability

General Stability Organism
benzamidine and PMSF stabilize during purification Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
dTDP cosubstrate ATP, 37°C, pH 7.6 Saccharomyces cerevisiae
0.25
-
dGDP cosubstrate ATP, 37°C, pH 7.6 Saccharomyces cerevisiae
0.26 0.37 CDP 37°C, pH 7.6 Saccharomyces cerevisiae
0.26 0.37 CDP cosubstrate ATP Saccharomyces cerevisiae
0.28
-
UDP cosubstrate ATP, 37°C, pH 7.6 Saccharomyces cerevisiae
0.5 0.54 dCDP cosubstrate ATP, 37°C, pH 7.6 Saccharomyces cerevisiae
0.5 0.54 dUDP cosubstrate ATP, 37°C, pH 7.6 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000 70000 FPLC gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NDP + NTP Saccharomyces cerevisiae involved in RNA- and DNA metabolism NTP + NDP
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
baker's yeast, strain BJ2168
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4
-
37°C, pH 7.6 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ADP less effective dinucleotide substrate Saccharomyces cerevisiae ADP + ATP
-
?
ATP + CDP highly effective dinucleotide substrate Saccharomyces cerevisiae ADP + CTP
-
?
ATP + dADP less effective dinucleotide substrate Saccharomyces cerevisiae ADP + dATP
-
?
ATP + dCDP less effective dinucleotide substrate Saccharomyces cerevisiae ADP + dCTP
-
?
ATP + dGDP less effective dinucleotide substrate Saccharomyces cerevisiae ADP + dGTP
-
?
ATP + dTDP highly effective dinucleotide substrate Saccharomyces cerevisiae ADP + dTTP
-
?
ATP + dUDP highly effective dinucleotide substrate Saccharomyces cerevisiae ?
-
?
ATP + UDP highly effective dinucleotide substrate Saccharomyces cerevisiae ADP + UTP
-
?
GTP + ADP GDP is less effective dinucleotide substrate Saccharomyces cerevisiae GDP + ATP
-
r
NDP + NTP involved in RNA- and DNA metabolism Saccharomyces cerevisiae NTP + NDP
-
?
NTP + nucleoside diphosphate broad specificity Saccharomyces cerevisiae NDP + NTP
-
?
NTP + nucleoside diphosphate in decreasing order of activity: UTP, dTTP, CTP, dATP, ATP, dGTP, GTP Saccharomyces cerevisiae NDP + NTP
-
?
UTP + dTDP best substrates Saccharomyces cerevisiae UDP + dTDP
-
?
UTP + dTDP nucleoside triphosphates in decreasing order of activity: dTTP, CTP, dCTP, dATP, dGTP, GTP Saccharomyces cerevisiae UDP + dTDP
-
?
UTP + dTDP nucleoside diphosphates in decreasing order of activity: CDP, UDP, GDP, dGDP, dCDP, dADP, ADP Saccharomyces cerevisiae UDP + dTDP
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 17000-18000, SDS-PAGE Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.3
-
dTDP
-
Saccharomyces cerevisiae