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Literature summary for 2.7.4.34 extracted from

  • Ishige, K.; Zhang, H.; Kornberg, A.
    Polyphosphate kinase (PPK2), a potent, polyphosphate-driven generator of GTP (2002), Proc. Natl. Acad. Sci. USA, 99, 16684-16688 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.34

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 GDP pH 7.2, 37°C Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Pseudomonas aeruginosa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ is favored over Mn2+ by 5fold at optimal concentration of 10 mM Pseudomonas aeruginosa
Mn2+ Mg2+ is favored over Mn2+ by 5fold at optimal concentration of 10 mM Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
164000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP + (phosphate)n+1 Pseudomonas aeruginosa
-
 GTP + (phosphate)n
-
 r
GDP + (phosphate)n+1 Pseudomonas aeruginosa DSM 22644
-
 GTP + (phosphate)n
-
 r
GTP + (phosphate)n Pseudomonas aeruginosa
-
 GDP + (phosphate)n+1
-
 r
GTP + (phosphate)n Pseudomonas aeruginosa DSM 22644
-
 GDP + (phosphate)n+1
-
 r

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I6Z1 cf. EC 2.7.4.1
-
Pseudomonas aeruginosa DSM 22644 Q9I6Z1 cf. EC 2.7.4.1
-

Purification (Commentary)

Purification (Comment) Organism
purified 1300fold to homogeneity from lysates Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
474
-
 synthesis of polyphosphate, pH 7.2, 37°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + (phosphate)n+1
-
 Pseudomonas aeruginosa GTP + (phosphate)n
-
 r
GDP + (phosphate)n+1
-
 Pseudomonas aeruginosa DSM 22644 GTP + (phosphate)n
-
 r
GTP + (phosphate)n
-
 Pseudomonas aeruginosa GDP + (phosphate)n+1
-
 r
GTP + (phosphate)n
-
 Pseudomonas aeruginosa DSM 22644 GDP + (phosphate)n+1
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 44000, SDS-PAGE, 4 * 40786, calculated from sequence Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
PA0141
-
 Pseudomonas aeruginosa
PPK2
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 30 min, 50% loss of activity Pseudomonas aeruginosa
50
-
 30 min, 96% loss of activity. With polyphospate present, the activity is fully retained even after 30 min at 50°C Pseudomonas aeruginosa

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas aeruginosa calculated from sequence
-
 9.9

Expression

Organism Comment Expression
Pseudomonas aeruginosa PPK2 appears in the stationary phase of growth and reaches nucleoside diphosphate kinase levels of 5-10% that of the classic nucleoside diphosphate kinase additional information

General Information

General Information Comment Organism
metabolism enzyme uses inorganic polyphosphate as a donor to convert GDP to GTP. Polyphosphate chains of 30-50 residues are optimal, those of 15-700 residues can also serve. The rate of polyphosphate utilization is nearly 100fold greater than that of polyphosphate synthesis Pseudomonas aeruginosa