KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
AMP | forward reaction of ADP synthesis, pH 8.0, 37°C | Acinetobacter johnsonii | |
8.3 | - |
ADP | reverse reaction of polyphosphate synthesis, pH 8.0, 37°C | Acinetobacter johnsonii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, best at 100 mM for polyphosphate utilization/ADP synthesis and 20 mM for polyphosphate synthesis. The equilibrium ratio for the AMP and ADP formation by PAP is 1:19 in the presence of 100 mM MgCl2 and is changed to 1:9.6, i.e. 9.4% of 5 mM ADP is converted to AMP, in the presence of 20 mM MgCl2 | Acinetobacter johnsonii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + [phosphate]n | Acinetobacter johnsonii | - |
AMP + [phosphate]n+1 | - |
r | |
ADP + [phosphate]n | Acinetobacter johnsonii 210A | - |
AMP + [phosphate]n+1 | - |
r | |
AMP + [phosphate]n | Acinetobacter johnsonii | - |
ADP + [phosphate]n-1 | - |
r | |
AMP + [phosphate]n | Acinetobacter johnsonii 210A | - |
ADP + [phosphate]n-1 | - |
r | |
GDP + [phosphate]n | Acinetobacter johnsonii | - |
GMP + [phosphate]n+1 | - |
r | |
GDP + [phosphate]n | Acinetobacter johnsonii 210A | - |
GMP + [phosphate]n+1 | - |
r | |
GMP + [phosphate]n | Acinetobacter johnsonii | - |
GDP + [phosphate]n-1 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter johnsonii | Q83XD3 | gene pap | - |
Acinetobacter johnsonii 210A | Q83XD3 | gene pap | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + [phosphate]n | - |
Acinetobacter johnsonii | AMP + [phosphate]n+1 | - |
r | |
ADP + [phosphate]n | - |
Acinetobacter johnsonii 210A | AMP + [phosphate]n+1 | - |
r | |
AMP + [phosphate]n | - |
Acinetobacter johnsonii | ADP + [phosphate]n-1 | - |
r | |
AMP + [phosphate]n | best substrate | Acinetobacter johnsonii | ADP + [phosphate]n-1 | - |
? | |
AMP + [phosphate]n | - |
Acinetobacter johnsonii 210A | ADP + [phosphate]n-1 | - |
r | |
AMP + [phosphate]n | best substrate | Acinetobacter johnsonii 210A | ADP + [phosphate]n-1 | - |
? | |
CMP + [phosphate]n | low activity | Acinetobacter johnsonii | CDP + [phosphate]n-1 | - |
? | |
GDP + [phosphate]n | - |
Acinetobacter johnsonii | GMP + [phosphate]n+1 | - |
r | |
GDP + [phosphate]n | - |
Acinetobacter johnsonii 210A | GMP + [phosphate]n+1 | - |
r | |
GMP + [phosphate]n | - |
Acinetobacter johnsonii | GDP + [phosphate]n-1 | - |
? | |
GMP + [phosphate]n | - |
Acinetobacter johnsonii | GDP + [phosphate]n-1 | - |
r | |
additional information | the forward reaction of polyphosphate utilization is preferred compared to the reverse reaction of polyphosphate synthesis. ADP is preferred over GDP as a phosphate donor for poly(P) synthesis. PAP of Acinetobacter johnsonii strain 210A is a type of poly(P) kinase that uses ADP and GDP as substrates, cf. EC 2.7.4.1. Analysis of the reversibility of the PAP reaction. The equilibrium ratio for the AMP and ADP formation by PAP is 1:19 in the presence of 100 mM MgCl2 and is changed to 1:9.6, i.e. 9.4% of 5 mM ADP is converted to AMP, in the presence of 20 mM MgCl2 | Acinetobacter johnsonii | ? | - |
? | |
additional information | the forward reaction of polyphosphate utilization is preferred compared to the reverse reaction of polyphosphate synthesis. ADP is preferred over GDP as a phosphate donor for poly(P) synthesis. PAP of Acinetobacter johnsonii strain 210A is a type of poly(P) kinase that uses ADP and GDP as substrates, cf. EC 2.7.4.1. Analysis of the reversibility of the PAP reaction. The equilibrium ratio for the AMP and ADP formation by PAP is 1:19 in the presence of 100 mM MgCl2 and is changed to 1:9.6, i.e. 9.4% of 5 mM ADP is converted to AMP, in the presence of 20 mM MgCl2 | Acinetobacter johnsonii 210A | ? | - |
? | |
UMP + [phosphate]n | low activity | Acinetobacter johnsonii | UDP + [phosphate]n-1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphosphate:AMP phosphotransferase | - |
Acinetobacter johnsonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Acinetobacter johnsonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Acinetobacter johnsonii |