Application | Comment | Organism |
---|---|---|
synthesis | PPT catalyzes a key reaction in the cell-free regeneration of ATP from AMP and polyphosphate. The PPT/AdK system provides an alternative to existing enzymatic ATP regeneration systems in which phosphoenolpyruvate and acetylphosphate serve as phosphoryl donors and has the advantage that AMP and polyP are stable, inexpensive substrates | Acinetobacter johnsonii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Acinetobacter johnsonii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + [phosphate]n | Acinetobacter johnsonii | - |
ADP + [phosphate]n-1 | - |
? | |
AMP + [phosphate]n | Acinetobacter johnsonii 210A | - |
ADP + [phosphate]n-1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter johnsonii | - |
- |
- |
Acinetobacter johnsonii 210A | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-dAMP + (phosphate)n | - |
Acinetobacter johnsonii | 2'-dADP + (phosphate)n-1 | - |
? | |
2'-dAMP + (phosphate)n | - |
Acinetobacter johnsonii 210A | 2'-dADP + (phosphate)n-1 | - |
? | |
2'-deoxy-AMP + [phosphate]n | - |
Acinetobacter johnsonii | 2'-deoxy-ADP + [phosphate]n-1 | - |
? | |
AMP + (phosphate)n | - |
Acinetobacter johnsonii | ADP + (phosphate)n-1 | - |
? | |
AMP + (phosphate)n | - |
Acinetobacter johnsonii 210A | ADP + (phosphate)n-1 | - |
? | |
AMP + [phosphate]n | - |
Acinetobacter johnsonii | ADP + [phosphate]n-1 | - |
? | |
AMP + [phosphate]n | - |
Acinetobacter johnsonii 210A | ADP + [phosphate]n-1 | - |
? | |
additional information | GMP, UMP, CMP and IMP are not converted to the corresponding diphosphates | Acinetobacter johnsonii | ? | - |
? | |
additional information | PPT substrate specificity, overview. GMP, UMP, CMP, and IMP are not converted to the corresponding diphosphates at significant rates | Acinetobacter johnsonii | ? | - |
? | |
additional information | GMP, UMP, CMP and IMP are not converted to the corresponding diphosphates | Acinetobacter johnsonii 210A | ? | - |
? | |
additional information | PPT substrate specificity, overview. GMP, UMP, CMP, and IMP are not converted to the corresponding diphosphates at significant rates | Acinetobacter johnsonii 210A | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphosphate:AMP phosphotransferase | - |
Acinetobacter johnsonii |
PPT | - |
Acinetobacter johnsonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Acinetobacter johnsonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Acinetobacter johnsonii |
General Information | Comment | Organism |
---|---|---|
additional information | ATP regeneration from AMP and polyphophate using PPT, firefly luciferase and hexokinase as model ATP-requiring enzymes, ADP can be converted to ATP by adenylate kinase, AdK. Establishment of a PPT/AdK system for ATP regeneration with coupled hexokinase, overview | Acinetobacter johnsonii |
physiological function | PPT from Acinetobacter johnsonii is specific for AMP and 2'-dAMP | Acinetobacter johnsonii |