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Literature summary for 2.7.4.3 extracted from
- Isolation and characterization of an adenylate kinase from the lateral muscle of bastard halibut Paralichthys olivaceus (2020), Fish. Sci., 86, 385-394 .
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 100 mM, optimal concentration | Paralichthys olivaceus |  |
| K+ | 150 mM, optimal concentration | Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
SDS-PAGE, gel filtration | Paralichthys olivaceus |
| 22000 | - |
SDS-PAGE, gel filtration | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | P05081 | - |
- |
| Paralichthys olivaceus | XP_019937160.1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| column chromatographies on TOYOPEARL SP-650 M and Superdex 75 10/300 | Paralichthys olivaceus |
| column chromatographies on TOYOPEARL SP-650 M and Superdex 75 10/300 | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | breast muscle | Gallus gallus | - |
| muscle | lateral muscle | Paralichthys olivaceus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ADP | - |
Paralichthys olivaceus | ATP + AMP | - |
r | |
| 2 ADP | - |
Gallus gallus | ATP + AMP | - |
r | |
| ATP + AMP | - |
Gallus gallus | 2 ADP | - |
r | |
| ATP + AMP | the molar ratio for ATP:ADP:AMP in the equilibrium state of the reaction is about 1:1:1 | Paralichthys olivaceus | 2 ADP | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 22000, SDS-PAGE | Paralichthys olivaceus |
| monomer | 1 * 22000, SDS-PAGE | Gallus gallus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Paralichthys olivaceus |
| 50 | 60 | - |
Gallus gallus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | 20°C: about 40% of maximal activity, 50°C: about 90% of maximal activity | Paralichthys olivaceus |
| 30 | 60 | 30°C: about 45% of maximal activity, 50-60°C: maximal activity, 70°C: about 10% of maximal activity | Gallus gallus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 46 | - |
30 min, 50% inactivation | Gallus gallus |
| 54 | - |
30 min, 50% inactivation | Paralichthys olivaceus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Paralichthys olivaceus |
| 7 | - |
- |
Gallus gallus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10.5 | 0°C, 3 h, less than 50% loss of activity | Paralichthys olivaceus |
| 4.5 | 8.5 | 0°C, 3 h, less than 40% loss of activity | Gallus gallus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme catalyzes a principal step in adenine nucleotide metabolism and cellular energy homeostasis | Paralichthys olivaceus |
| physiological function | the enzyme catalyzes a principal step in adenine nucleotide metabolism and cellular energy homeostasis | Gallus gallus |
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