Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Clonorchis sinensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
ADP | in 100 mM TrisHCl (pH 7.5), 20 mM glucose, 5 mM MgCl2, 10 mM KCl, 2 mM dithiothreitol, at 37°C | Clonorchis sinensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme is slightly activated by Ca2+ (85% relative activity at 5 mM) | Clonorchis sinensis | |
K+ | the enzyme is highly activated by K+, the optimal concentration is 10 mM | Clonorchis sinensis | |
Li+ | the enzyme is slightly activated by Li+ +(65.87% relative activity at 10 mM) | Clonorchis sinensis | |
Mg2+ | the enzyme activity is highly dependent on Mg2+, and the optimal concentration of Mg2+ is 2 mM | Clonorchis sinensis | |
additional information | the enzyme is not activated by Mn2+ | Clonorchis sinensis | |
NH4+ | the enzyme is highly activated by NH4+ (93.6% relative activity at 5 mM) | Clonorchis sinensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26700 | - |
x * 26700, His12-tagged enzyme ADK1, estimated from SDS-PAGE | Clonorchis sinensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clonorchis sinensis | Q8MVS0 | - |
- |
Purification (Comment) | Organism |
---|---|
His Bind column chromatography | Clonorchis sinensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | - |
Clonorchis sinensis | - |
egg | - |
Clonorchis sinensis | - |
metacercaria | - |
Clonorchis sinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + ADP | - |
Clonorchis sinensis | ATP + AMP | - |
r | |
ATP + AMP | - |
Clonorchis sinensis | ADP + ADP | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 26700, His12-tagged enzyme ADK1, estimated from SDS-PAGE | Clonorchis sinensis |
Synonyms | Comment | Organism |
---|---|---|
adenylate kinase 1 | - |
Clonorchis sinensis |
ADK1 | - |
Clonorchis sinensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Clonorchis sinensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Clonorchis sinensis |
Organism | Comment | Expression |
---|---|---|
Clonorchis sinensis | the expression level of ADK1 at the stage of excysted metacercaria is higher than those of adult worm (18.8fold), metacercariae (1.5fold) and eggs (5.6fold) | up |