Literature summary for 2.7.4.3 extracted from

Gavel, O.Y.; Bursakov, S.A.; Di Rocco, G.; Trincao, J.; Pickering, I.J.; George, G.N.; Calvete, J.J.; Shnyrov, V.L.; Brondino, C.D.; Pereira, A.S.; Lampreia, J.; Tavares, P.; Moura, J.J.; Moura, I.
A new type of metal-binding site in cobalt- and zinc-containing adenylate kinases isolated from sulfate-reducers Desulfovibrio gigas and Desulfovibrio desulfuricans ATCC 27774 (2008), J. Inorg. Biochem., 102, 1380-1395.

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Inhibitors

Inhibitors	Comment	Organism	Structure
KCl	no inhibitory up to 150 mM, 65% residual activity at 700 mM	Desulfovibrio desulfuricans
KCl	not inhibitory up to 150 mM, 65% residual activity at 700 mM	Megalodesulfovibrio gigas

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cobalt	0.3 mol of cobalt and 0.1 mol of zinc per mol of protein	Desulfovibrio desulfuricans
Cobalt	0.4 mol of cobalt and 0.3 mol of zinc per mol of protein. Presence of three sulfydryl groups of cysteines potentially bound to Co2+ or Zn2+. Bound Zn2+ or Co2+ is clearly present in the LID domain and tetrahedrally coordinated to 129Cys, 135His, 151Cys, and 154Cys. Site 129Cys-X5-His-X15-Cys-X2-Cys is responsible for chelating zinc or cobalt	Megalodesulfovibrio gigas
Zinc	0.3 mol of cobalt and 0.1 mol of zinc per mol of protein	Desulfovibrio desulfuricans
Zinc	0.4 mol of cobalt and 0.3 mol of zinc per mol of protein. Presence of three sulfydryl groups of cysteines potentially bound to Co2+ or Zn2+. Bound Zn2+ or Co2+ is clearly present in the LID domain and tetrahedrally coordinated to 129Cys, 135His, 151Cys, and 154Cys. Site 129Cys-X5-His-X15-Cys-X2-Cys is responsible for chelating zinc or cobalt	Megalodesulfovibrio gigas

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24500	-	1 * 24500, SDS-PAGE, 1 * 24700, electrospray mass spectrometry, 1 * 24500, calculated	Megalodesulfovibrio gigas
24700	-	1 * 24500, SDS-PAGE, 1 * 24700, electrospray mass spectrometry, 1 * 24500, calculated	Megalodesulfovibrio gigas
24800	-	1 * 25500, SDS-PAGE, 1 * 24800, electrospray mass spectrometry	Desulfovibrio desulfuricans
25500	-	1 * 25500, SDS-PAGE, 1 * 24800, electrospray mass spectrometry	Desulfovibrio desulfuricans
26000	-	gel filtration	Desulfovibrio desulfuricans
26000	-	gel filtration	Megalodesulfovibrio gigas

Organism

Organism	UniProt	Comment	Textmining
Desulfovibrio desulfuricans	-	-	-
Megalodesulfovibrio gigas	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1560	-	apoenzyme, pH 7.4, 30°C	Megalodesulfovibrio gigas
1640	-	ATP formation, pH 7.4, 30°C	Desulfovibrio desulfuricans
1980	-	fully zinc-substitued form, pH 7.4, 30°C	Megalodesulfovibrio gigas
2020	-	native form, pH 7.4, 30°C	Megalodesulfovibrio gigas
2170	-	fully cobalt-substitued form, pH 7.4, 30°C	Megalodesulfovibrio gigas

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP	-	Desulfovibrio desulfuricans	AMP + ATP	-	r
ADP	-	Megalodesulfovibrio gigas	AMP + ATP	-	r

Subunits

Subunits	Comment	Organism
monomer	1 * 24500, SDS-PAGE, 1 * 24700, electrospray mass spectrometry, 1 * 24500, calculated	Megalodesulfovibrio gigas
monomer	1 * 25500, SDS-PAGE, 1 * 24800, electrospray mass spectrometry	Desulfovibrio desulfuricans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	ADP formation	Desulfovibrio desulfuricans

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.8	6.5	direction of ADP formation	Megalodesulfovibrio gigas
7.5	9	direction of ATP formation	Megalodesulfovibrio gigas

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4.8	11	stable within	Desulfovibrio desulfuricans
4.8	11	stable within	Megalodesulfovibrio gigas

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Release 2023.1 (January 2023)