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Literature summary for 2.7.4.3 extracted from
- Adenylate kinase and GTP:AMP phosphotransferase of the malarial parasite Plasmodium falciparum. Central players in cellular energy metabolism (2004), Mol. Biochem. Parasitol., 136, 211-220.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain M15 | Plasmodium falciparum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: atractyloside, chloroquine, primaquine, artemisinine, mefloquine | Plasmodium falciparum | |
| P1,P5-di(adenosine-5')pentaphosphate | - |
Plasmodium falciparum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
AMP | pH 6.0 and pH 7.4 | Plasmodium falciparum | |
| 0.12 | - |
ATP | pH 7.4 | Plasmodium falciparum | |
| 0.13 | - |
ATP | pH 6.0 | Plasmodium falciparum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27600 | - |
calculated without His-tag | Plasmodium falciparum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | Q7Z0H0 | strains HB3 and Dd2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Plasmodium falciparum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Vmax 75 1 mM at pH 6.0 and 25°C | Plasmodium falciparum |
| 100 | - |
wild type AK | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + AMP | - |
Plasmodium falciparum | ADP + ADP | - |
? | |
| CTP + AMP | - |
Plasmodium falciparum | CDP + ADP | - |
? | |
| GTP + AMP | - |
Plasmodium falciparum | GDP + ADP | - |
? | |
| ITP + AMP | - |
Plasmodium falciparum | IDP + ADP | - |
? | |
| additional information | monophosphates: IMP, GMP, CMP, UMP: activity below 1% | Plasmodium falciparum | ? | - |
? | |
| UTP + AMP | - |
Plasmodium falciparum | UDP + ADP | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
P1,P5-di(adenosine-5')pentaphosphate | pH 7.4 | Plasmodium falciparum | |
| 0.00038 | - |
P1,P5-di(adenosine-5')pentaphosphate | pH 6 | Plasmodium falciparum | |
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