Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
P1,P5-(bis adenosine)-5'-pentaphosphate | inhibited activity of the recombinant enzyme, also inhibited the growth of L. donovani promastigotes in vitro | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.074 | - |
AMP | pH 8 | Leishmania donovani | |
0.104 | - |
ATP | pH 8 | Leishmania donovani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
calculated from nucleotide sequence | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | Q9Y0E7 | 1S2D strain | - |
Leishmania donovani 1S2D | Q9Y0E7 | 1S2D strain | - |
Purification (Comment) | Organism |
---|---|
- |
Leishmania donovani |
Renatured (Comment) | Organism |
---|---|
refolding of urea-denatured adenylate kinase by equilibrium dialysis against 50 mM Tris/HCl pH 8.0, containing either MgSO4, KCl or 10 mM dithiothreitol | Leishmania donovani |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
promastigote | - |
Leishmania donovani | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Vmax 0.00018 pH 8 | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + AMP | - |
Leishmania donovani | ADP + ADP | - |
? | |
ATP + AMP | - |
Leishmania donovani 1S2D | ADP + ADP | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
t1/2: 29 h | Leishmania donovani |
13 | - |
t1/2: 24 h | Leishmania donovani |
26.5 | - |
t1/2: 12h | Leishmania donovani |