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Literature summary for 2.7.4.3 extracted from
- AMP (dAMP) kinase from human erythrocytes (1978), Methods Enzymol., 51, 467-473.
General Stability
| General Stability | Organism |
|---|---|
| Triton X-100, EDTA, dithiothreitol and electrolyte protect enzyme in dilute solution and against denaturation by heat or extreme pH-values | Homo sapiens |
| unstable in dilute solution | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.076 | 0.083 | AMP | 25°C, pH 8.0 | Homo sapiens |  |
| 0.09 | 0.092 | ADP3- | 25°C, pH 8.0 | Homo sapiens | |
| 0.09 | 0.092 | ADP3- | ATP production | Homo sapiens | |
| 0.11 | - |
ADP | 25°C, pH 8.0 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| additional information | stroma-associated | Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21300 | - |
sedimentation equilibrium | Homo sapiens |
| 23000 | - |
1 * 23000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 allelozymes: AK1*1 and AK1*2 | Homo sapiens |
| and their multiple forms | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3200 | - |
25°C, pH 8.0 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + ADP | - |
Homo sapiens | ATP + AMP | - |
r | |
| ATP + AMP | no substrates are ATP/dGMP | Homo sapiens | ADP + ADP | - |
r | |
| ATP + AMP | no substrates are ATP/IMP | Homo sapiens | ADP + ADP | - |
r | |
| ATP + AMP | no substrates are dGTP/AMP | Homo sapiens | ADP + ADP | - |
r | |
| ATP + AMP | no substrates are ATP/UMP | Homo sapiens | ADP + ADP | - |
r | |
| ATP + AMP | no substrates are ATP/GMP | Homo sapiens | ADP + ADP | - |
r | |
| ATP + CMP | reaction at 1% the rate of AMP | Homo sapiens | ADP + ? | - |
? | |
| ATP + dAMP | reaction at 10% the rate of AMP | Homo sapiens | ADP + dADP | - |
? | |
| CTP + AMP | reaction at about 3% the rate of ATP | Homo sapiens | ADP + CDP | - |
? | |
| dATP + AMP | reaction at about 50% the rate of ATP | Homo sapiens | dADP + ADP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 23000, SDS-PAGE | Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | - |
- |
9 |
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Release 2023.1 (January 2023)
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