Any feedback?
Literature summary for 2.7.4.3 extracted from
- AMP metabolism by the marine bacterium Vibrio (Benecka) natriegens: purification and properties of adenylate kinase (1981), Can. J. Microbiol., 27, 1053-1059.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol and 2-mercaptoethanol are not necessary as protecting agents | Vibrio natriegens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Vibrio natriegens |  |
| IAA | - |
Vibrio natriegens | |
| N-ethylmaleimide | weak | Vibrio natriegens | |
| p-chloromercuriphenylsulfonate | - |
Vibrio natriegens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
ATP | 30°C, pH 7.4 | Vibrio natriegens | |
| 0.114 | 0.13 | AMP | - |
Vibrio natriegens | |
| 0.45 | 0.55 | ADP | 30°C, pH 7.4 | Vibrio natriegens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Vibrio natriegens | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Vibrio natriegens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
gel filtration | Vibrio natriegens |
| 32000 | - |
1 * 32000, SDS-PAGE | Vibrio natriegens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio natriegens | - |
Beneckea, type III | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vibrio natriegens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1450 | - |
30°C, pH 7.4 | Vibrio natriegens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + ADP | - |
Vibrio natriegens | ATP + AMP | - |
r | |
| ATP + AMP | no substrates are ATP/IMP | Vibrio natriegens | ADP + ADP | - |
r | |
| ATP + AMP | no substrates are ATP/UMP | Vibrio natriegens | ADP + ADP | - |
r | |
| ATP + AMP | no substrates are ATP/GMP | Vibrio natriegens | ADP + ADP | - |
r | |
| ATP + dAMP | reaction at 46% the rate of AMP | Vibrio natriegens | ADP + dADP | - |
? | |
| dATP + AMP | reaction at about 50% the rate of ATP | Vibrio natriegens | dADP + ADP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32000, SDS-PAGE | Vibrio natriegens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Vibrio natriegens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 325 | - |
ATP | - |
Vibrio natriegens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.6 | broad | Vibrio natriegens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | less than 20% of maximal activity at pH 5, 5.5, 6 and pH 9.5 and 10, about half-maximal activity at pH 6.9 and 8.4 | Vibrio natriegens |
| 6.9 | 8.4 | about half-maximal activity at pH 6.9 and 8.4 | Vibrio natriegens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Vibrio natriegens | isoelectric focusing | 4.8 | 4.7 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
