Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglyceraldehyde | - |
Starkeya novella | |
acetyl-CoA | - |
Starkeya novella | |
AMP | product inhibition | Starkeya novella | |
ATP | product inhibition | Starkeya novella | |
CoA | - |
Starkeya novella | |
diphosphate | reverse reaction | Starkeya novella | |
Hg2+ | strong, not reaction of ADP + ADP | Starkeya novella | |
Mg2+ | above 0.5 mM | Starkeya novella | |
additional information | effect of varios intermediary metabolites; no inhibition by K+, Na+, NH4+, AsO2, citrate, NADH, fructose 6-phosphate, 2-phosphoglyceraldehyde | Starkeya novella | |
N-ethylmaleimide | not | Starkeya novella | |
p-hydroxymercuribenzoate | strong, reversible by GSH or cysteine | Starkeya novella | |
phosphoenolpyruvate | - |
Starkeya novella |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | 0.04 | AMP | pH 7.5, 25°C, ADP production | Starkeya novella | |
0.09 | 0.092 | ADP3- | pH 8.7, 25°C | Starkeya novella | |
0.09 | 0.092 | ADP3- | ATP production | Starkeya novella | |
0.34 | 0.35 | ADP | pH 8.7, 25°C | Starkeya novella |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Starkeya novella | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | about 50% as effective as Mg2+ | Starkeya novella | |
Mg2+ | no absolute requirement: 20% of activity in its absence | Starkeya novella | |
Mn2+ | requirement, about 25% as effective as Mg2+ | Starkeya novella |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
sucrose density gradient centrifugation | Starkeya novella |
46300 | - |
1 * 46300, SDS-PAGE | Starkeya novella |
47800 | - |
1 * 47800, SDS-PAGE after treatment with 5 M urea | Starkeya novella |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + ADP | Starkeya novella | involved in energy metabolism | ? | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Starkeya novella | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Starkeya novella |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32 | - |
pH 8.7, 25°C, ATP production | Starkeya novella |
Storage Stability | Organism |
---|---|
-20°C, several weeks | Starkeya novella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + ADP | no substrate: CDP | Starkeya novella | ATP + AMP | - |
r | |
ADP + ADP | no substrates: IDP, GDP | Starkeya novella | ATP + AMP | - |
r | |
ADP + ADP | involved in energy metabolism | Starkeya novella | ? | - |
r | |
ATP + AMP | no substrates are adenosine, 2',3'-AMP or 3',5'-AMP | Starkeya novella | ADP + ADP | - |
r | |
ATP + AMP | no substrate of the reverse reaction: CDP | Starkeya novella | ADP + ADP | - |
r | |
ATP + AMP | no substrates are ATP/IMP | Starkeya novella | ADP + ADP | - |
r | |
ATP + AMP | no substrates of the reverse reaction: IDP, GDP | Starkeya novella | ADP + ADP | - |
r | |
ATP + AMP | specific for ATP, AMP and ADP | Starkeya novella | ADP + ADP | - |
r | |
ATP + AMP | no substrates are ATP/GMP | Starkeya novella | ADP + ADP | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46300, SDS-PAGE | Starkeya novella |
monomer | 1 * 47800, SDS-PAGE after treatment with 5 M urea | Starkeya novella |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, inactivation, 0.5 mM dithiothreitol protects | Starkeya novella |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
AMP + ATP | Starkeya novella |
8.7 | - |
ADP + ADP | Starkeya novella |