Crystallization (Comment) | Organism |
---|---|
liver | Homo sapiens |
muscle | Homo sapiens |
General Stability | Organism |
---|---|
stable to dialysis in the presence of 4 mM dithioerythritol | Homo sapiens |
Stable to freeze-thawing | Homo sapiens |
unstable in dilute solution, 0.2 mg/ml, dithioerythritol, and bovine serum albumin stabilize | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25200 | - |
liver, sedimentation equilibrium | Homo sapiens |
26349 | - |
1 * 26349, calculated from amino acid analysis | Homo sapiens |
26350 | - |
calculated from amino acid analysis | Homo sapiens |
26500 | - |
1 * 26500, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
post mortem | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
muscle | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
280 | - |
30°C, pH 7.4 | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, several weeks | Homo sapiens |
3°C, 5-10% loss within 1 month | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine 5'-(3-thio)triphosphate + AMP | muscle: reaction at 97% the rate of ATP, liver mitochondria: reaction at 70% the rate of ATP | Homo sapiens | adenosine 5'-diphosphate + adenosine 5'-(3-thio)diphosphate | - |
? | |
ADP + ADP | no substrates: adenosine 5'-(2-thio)diphosphate, adenosine diphosphate 3'-diphosphate | Homo sapiens | ATP + AMP | - |
? | |
ATP + adenosine 5'-thiophosphate | muscle: reaction at 56% the rate of AMP, liver mitochondria: reaction at 95% the rate of AMP | Homo sapiens | ? | - |
? | |
ATP + AMP | no substrates are adenosine triphosphate 3'-diphosphate, adenosine-5'-(3-thio)triphosphate/adenosine 5'-thiophosphate | Homo sapiens | ADP + ADP | - |
r | |
ATP + AMP | no substrates of the reverse reaction: adenosine 5'-(2-thio)diphosphate, adenosine diphosphate 3'-diphosphate | Homo sapiens | ADP + ADP | - |
r | |
ATP + AMP-3'-diphosphate | muscle: reaction at 57% the rate of AMP, liver mitochondria: reaction at 86% the rate of AMP | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 26500, SDS-PAGE | Homo sapiens |
monomer | 1 * 26349, calculated from amino acid analysis | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
AMP + ATP | Homo sapiens |
7.9 | - |
ADP + ADP | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.2 | about half-maximal activity at pH 6.5 and 8.2 | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
1 h, at 0°C, quite stable | Homo sapiens |
11.5 | - |
1 h, at 0°C, quite stable | Homo sapiens |