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Literature summary for 2.7.4.27 extracted from
- Regulation of C4 photosynthesis: Purification and properties of the protein catalyzing ADP-mediated inactivation and Pi-mediated activation of pyruvate,Pi dikinase (1985), Arch. Biochem. Biophys., 237, 490-503.
General Stability
| General Stability | Organism |
|---|---|
| stabilized at 25°C by phosphate, ATP, Blue Dextran and Cibacron blue | Zea mays |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) | Zea mays |  |
| ADP-beta-S | competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) | Zea mays | |
| AMP | competitive to phosphate | Zea mays | |
| CDP | - |
Zea mays | |
| Cibacron blue | competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) | Zea mays | |
| diphosphate | competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) | Zea mays | |
| GDP | - |
Zea mays | |
| pyruvate,Pi dikinase phosphorylated at a His residue | competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) | Zea mays |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0007 | - |
[pyruvate,Pi dikinase]phosphate | phosphorylated at a Thr residue, pH 8.3, temperature not specified in the publication | Zea mays | |
| 0.65 | - |
phosphate | pH 8.3, temperature not specified in the publication | Zea mays | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Zea mays |
| 90000 | - |
pH 7.5, gel filtration | Zea mays |
| 180000 | - |
pH 8.3, gel filtration | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
cv. Dekalb XL81 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, dye ligand chromatography (agarose-Blue Dextran), gel filtration | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [pyruvate,Pi dikinase]phosphate + phosphate | phosphorylated at a Thr residue, inactive, additionally catalytically phosphorylated substrate (at His residue) is a much poorer substrate | Zea mays | pyruvate,Pi dikinase + diphosphate | active | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Zea mays |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pyruvate,Pi dikinase regulatory protein | bifunctional, catalyses phosphorylation and dephosphorylation of pyruvate,Pi dikinase | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
broad optimum pH 7.8-8.4, 68% activity at pH 7.2 | Zea mays |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
Cibacron blue | pH 8.3, temperature not specified in the publication | Zea mays | |
| 0.08 | - |
ADP-beta-S | Ki-value below 0.08 mM, pH 8.3, temperature not specified in the publication | Zea mays | |
| 0.085 | - |
ADP | pH 8.3, temperature not specified in the publication | Zea mays | |
| 0.16 | - |
diphosphate | pH 8.3, temperature not specified in the publication | Zea mays | |
| 0.4 | - |
AMP | pH 8.3, temperature not specified in the publication | Zea mays | |
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