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Literature summary for 2.7.4.26 extracted from

  • Mabanglo, M.F.; Serohijos, A.W.; Poulter, C.D.
    The Streptomyces-produced antibiotic fosfomycin is a promiscuous substrate for archaeal isopentenyl phosphate kinase (2012), Biochemistry, 52, 917-925.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.26

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Thermoplasma acidophilum
-
 Streptomyces wedmorensis

Protein Variants

Protein Variants Comment Organism
K204A kinetic values similar to wild-type Thermoplasma acidophilum

Inhibitors

Inhibitors Comment Organism Structure
fosfomycin competitive versus isopentenyl phosphate Streptomyces wedmorensis
fosfomycin competitive versus isopentenyl phosphate Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 isopentenyl phosphate pH 7.5, 37°C, mutant K204A Thermoplasma acidophilum
0.0044
-
 isopentenyl phosphate pH 7.5, 37°C, wild-type Thermoplasma acidophilum
0.006
-
 ATP pH 7.5, 37°C, wild-type Thermoplasma acidophilum
0.0069
-
 ATP pH 7.5, 37°C, mutant K204A Thermoplasma acidophilum
0.11
-
 fosfomycin pH 7.5, 37°C, mutant K204A Thermoplasma acidophilum
0.15
-
 fosfomycin pH 7.5, 37°C, wild-type Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + isopentenyl phosphate Thermoplasma acidophilum
-
 ADP + isopentenyl diphosphate
-
 ?
ATP + isopentenyl phosphate Streptomyces wedmorensis
-
 ADP + isopentenyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces wedmorensis Q56187
-
-
Thermoplasma acidophilum Q9HLX1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + fosfomycin i.e. [(2R,3S)-3-methyl-2-oxiranyl]phosphonic acid Thermoplasma acidophilum ADP + ((2R,3S)-3-methyloxiran-2-yl)phosphonic phosphoric anhydride
-
 ?
ATP + fosfomycin i.e. [(2R,3S)-3-methyl-2-oxiranyl]phosphonic acid Streptomyces wedmorensis ADP + ((2R,3S)-3-methyloxiran-2-yl)phosphonic phosphoric anhydride
-
 ?
ATP + isopentenyl phosphate
-
 Thermoplasma acidophilum ADP + isopentenyl diphosphate
-
 ?
ATP + isopentenyl phosphate
-
 Thermoplasma acidophilum ADP + isopentenyl diphosphate
-
 r
ATP + isopentenyl phosphate
-
 Streptomyces wedmorensis ADP + isopentenyl diphosphate
-
 ?
ATP + isopentenyl phosphate
-
 Streptomyces wedmorensis ADP + isopentenyl diphosphate
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 fosfomycin pH 7.5, 37°C, mutant K204A Thermoplasma acidophilum
0.04
-
 fosfomycin pH 7.5, 37°C, wild-type Thermoplasma acidophilum
7.7
-
 isopentenyl phosphate pH 7.5, 37°C, mutant K204A Thermoplasma acidophilum
8
-
 isopentenyl phosphate pH 7.5, 37°C, wild-type Thermoplasma acidophilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0025
-
 fosfomycin pH 7.5, 37°C, mutant K204A Thermoplasma acidophilum
0.0026
-
 fosfomycin pH 7.5, 37°C, wild-type Thermoplasma acidophilum
1800
-
 isopentenyl phosphate pH 7.5, 37°C, wild-type Thermoplasma acidophilum
2800
-
 isopentenyl phosphate pH 7.5, 37°C, mutant K204A Thermoplasma acidophilum