Cloned (Comment) | Organism |
---|---|
into pSK50-delta A2 and then subcloned into pCAH56 and into pAH126 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21410 | - |
calculated from the deduced nucleotide sequence of the allele specifying the histidine-tailed polypeptide | Escherichia coli |
23500 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P16690 | K-12 strains | - |
Escherichia coli | P16690 | phnN | - |
Purification (Comment) | Organism |
---|---|
purification of histidine-tailed ribose 1,5-bisphosphokinase, centrifugation, ultrasonic treatment, application to Co2+-containing Talon metal affinity resin, purified almost to homogeneity | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
acitivity very low in a crude extract of strain HO1088/pTR553 before addition of IPTG | Escherichia coli |
0.03 | - |
acitivity in a crude extract of strain HO1088/pHO500 after addition of IPTG | Escherichia coli |
0.12 | - |
acitivity in a crude extract of strain HO1088/pTR553 after addition of IPTG | Escherichia coli |
8.3 | - |
purified PhnN protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ribose 1,5-bisphosphate | - |
Escherichia coli | 5-phospho-alpha-D-ribose 1-diphosphate + ADP | - |
? | |
additional information | no catalysis with ATP + ribose, ATP + ribose 1-phosphate or ATP + ribose 5-phosphate detected | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PhnN protein | - |
Escherichia coli |
ribose 1,5-bisphosphokinase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli | |
additional information | GTP cannot act as a phosphoryl donor | Escherichia coli |