Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | when 0.5 M GTP is added in the reaction mixture the affinity of the enzyme towards its UMP substrate decreases almost by 3folds. GTP increases the affinity of ATP towards the enzyme | Staphylococcus aureus | |
UTP | potent competitive inhibitor | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00276 | - |
UMP | purified enzyme, in 50 mM Tris-HCl pH 7.4, at 30°C | Staphylococcus aureus | |
0.00281 | - |
UMP | crude cytosolic extract, in 50 mM Tris-HCl pH 7.4, at 30°C | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Staphylococcus aureus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
6 * 25000, SDS-PAGE | Staphylococcus aureus |
150000 | - |
gel filtration | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | B8XPY6 | - |
- |
Staphylococcus aureus ATCC 12600 | B8XPY6 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE cellulose column chromatography, Sephadex G-200 gel filtration, and nickel metal chelate affinity chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | - |
Staphylococcus aureus | ADP + UDP | - |
? | |
ATP + UMP | - |
Staphylococcus aureus ATCC 12600 | ADP + UDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 25000, SDS-PAGE | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
UMPK | - |
Staphylococcus aureus |
uridine monophosphate kinase | - |
Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
UTP | in 50 mM Tris-HCl pH 7.4, at 30°C | Staphylococcus aureus |