Literature summary for 2.7.4.22 extracted from

Briozzo, P.; Evrin, C.; Meyer, P.; Assairi, L.; Joly, N.; Barzu, O.; Gilles, A.M.
Structure of Escherichia coli UMP kinase differs from that of other nucleoside monophosphate kinases and sheds new light on enzyme regulation (2005), J. Biol. Chem., 280, 25533-25540.

Search for more literature for 2.7.4.22

Activating Compound

Activating Compound	Comment	Organism	Structure
GTP	-	Escherichia coli

Application

Application	Comment	Organism
drug development	as bacterial UMP kinases have no counterpart in eukaryotes, the information provided here can help the design of new antibiotics	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21 (DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
bound to the UMP substrate, resolved at 2.3 A resolution, bound to the UDP product, resolved at 2.6 A resolution, bound to UTP, resolved at 2.45 A resolution	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
N140A	decreases half-denaturation temperature of UMP kinase by around 10°C, moderate loss of sensitivity to UTP inhibition, important loss in activation by GTP	Escherichia coli
T138A	decreases half-denaturation temperature of UMP kinase by around 10°C, results in 4times higher Km for UMP, moderate loss of sensitivity to UTP inhibition, important loss in activation by GTP	Escherichia coli
T138A/N140A	decreases half-denaturation temperature of UMP kinase by around 25°C, increases the apparant Km for ATP and UMP by a factor of 2.6 and 12, respectively	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
UTP	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	UMP	wild-type	Escherichia coli
0.056	-	UMP	mutant N140A	Escherichia coli
0.083	-	ATP	mutant N140A	Escherichia coli
0.16	-	ATP	wild-type	Escherichia coli
0.19	-	UMP	mutant T138A	Escherichia coli
0.29	-	ATP	mutant T138A	Escherichia coli
0.42	-	ATP	mutant T138A/N140A	Escherichia coli
0.6	-	UMP	mutant T138A/N140A	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + UMP	Escherichia coli	-	ADP + UDP	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A7E9	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
52	-	mutant N140A	Escherichia coli
88	-	mutant T138A	Escherichia coli
90	-	mutant T138A/N140A	Escherichia coli
96	-	wild-type	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + UMP	-	Escherichia coli	ADP + UDP	-	?
ATP + UMP	at 30°C, pH 7.4, in the presence of 2 mM MgCl2	Escherichia coli	ADP + UDP	-	?

Subunits

Subunits	Comment	Organism
hexamer	crystal structure	Escherichia coli

Synonyms

Synonyms	Comment	Organism
UMP kinase	-	Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
43	-	mutant T138A/N140A, temperature of half-inactivation	Escherichia coli
56	-	mutant T138A, temperature of half-inactivation	Escherichia coli
59	-	mutant N140A, temperature of half-inactivation	Escherichia coli
68	-	wild-type, temperature of half-inactivation	Escherichia coli

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Release 2023.1 (January 2023)