Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutant enzymes with a Flag-tag beta-adducin tail and MARCKS domain tagged to it, or tagged with actin-binding domain (1-275 aa), repeat domain (303-525 aa), and PH domain (2197-2307 aa) of beta2-spectrin. Tag cleavage by prescission protease | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
K217A | a kinase-dead mutant IP6K3, the catalytically inactive IP6K3 K217A mutant binds spectrin/adducin similarly to the wild-type enzyme | Mus musculus |
additional information | IP6K3 knockdown HEK293 cells are generated by lentiviral transduction. Diminished cerebellar synapses in IP6K3 mutants | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | enzyme IP6K3 physiologically binds to the cytoskeletal proteins adducin and spectrin in mouse cerebellum | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q8BWD2 | gene IP6K3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | immunohistochemic analysis of the enzyme in wild-type and mutant mouse brains, overview | Mus musculus | - |
cerebellum | - |
Mus musculus | - |
Purkinje cell | isozyme IP6K3 is highly concentrated in the brain in cerebellar Purkinje cells | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme IP6K3 physiologically binds to the cytoskeletal proteins adducin and spectrin in mouse cerebellum | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
inositol hexakisphosphate kinase-3 | - |
Mus musculus |
IP6K3 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | IP6K3 knock-out cerebella manifest abnormalities in Purkinje cell structure and synapse number, and the mutant mice display deficits in motor learning and coordination. Diminished cerebellar synapses in IP6K3 mutants | Mus musculus |
physiological function | inositol hexakisphosphate kinase-3 regulates the morphology and synapse formation of cerebellar Purkinje cells via spectrin/adducin, isozyme IP6K3 is a major determinant of cytoskeletal disposition and function of cerebellar Purkinje cells. Spectrin/adducin binding is substantially increased by IP6K3, independent of the enzyme's kinase activity, catalytically inactive IP6K3 K217A mutant binds spectrin/adducin similarly to the wild-type enzyme | Mus musculus |