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Literature summary for 2.7.4.21 extracted from

  • Fu, C.; Xu, J.; Li, R.J.; Crawford, J.A.; Khan, A.B.; Ma, T.M.; Cha, J.Y.; Snowman, A.M.; Pletnikov, M.V.; Snyder, S.H.
    Inositol hexakisphosphate kinase-3 regulates the morphology and synapse formation of cerebellar Purkinje cells via spectrin/adducin (2015), J. Neurosci., 35, 11056-11067.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.21

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant enzymes with a Flag-tag beta-adducin tail and MARCKS domain tagged to it, or tagged with actin-binding domain (1-275 aa), repeat domain (303-525 aa), and PH domain (2197-2307 aa) of beta2-spectrin. Tag cleavage by prescission protease Mus musculus

Protein Variants

Protein Variants Comment Organism
K217A a kinase-dead mutant IP6K3, the catalytically inactive IP6K3 K217A mutant binds spectrin/adducin similarly to the wild-type enzyme Mus musculus
additional information IP6K3 knockdown HEK293 cells are generated by lentiviral transduction. Diminished cerebellar synapses in IP6K3 mutants Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus enzyme IP6K3 physiologically binds to the cytoskeletal proteins adducin and spectrin in mouse cerebellum ?
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 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q8BWD2 gene IP6K3
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Source Tissue

Source Tissue Comment Organism Textmining
brain immunohistochemic analysis of the enzyme in wild-type and mutant mouse brains, overview Mus musculus
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cerebellum
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 Mus musculus
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Purkinje cell isozyme IP6K3 is highly concentrated in the brain in cerebellar Purkinje cells Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme IP6K3 physiologically binds to the cytoskeletal proteins adducin and spectrin in mouse cerebellum Mus musculus ?
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 ?

Synonyms

Synonyms Comment Organism
inositol hexakisphosphate kinase-3
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 Mus musculus
IP6K3
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 Mus musculus

General Information

General Information Comment Organism
malfunction IP6K3 knock-out cerebella manifest abnormalities in Purkinje cell structure and synapse number, and the mutant mice display deficits in motor learning and coordination. Diminished cerebellar synapses in IP6K3 mutants Mus musculus
physiological function inositol hexakisphosphate kinase-3 regulates the morphology and synapse formation of cerebellar Purkinje cells via spectrin/adducin, isozyme IP6K3 is a major determinant of cytoskeletal disposition and function of cerebellar Purkinje cells. Spectrin/adducin binding is substantially increased by IP6K3, independent of the enzyme's kinase activity, catalytically inactive IP6K3 K217A mutant binds spectrin/adducin similarly to the wild-type enzyme Mus musculus