malfunction |
deletion of KCS1, which blocks synthesis of inositol diphosphates on the 5-hydroxyl of the inositol ring, causes inositol auxotrophy and decreased intracellular inositol and phosphatidylinositol levels. These defects are caused by a profound decrease in transcription of INO1, which encodes myo-inositol-3-phosphate synthase. Expression of genes that function in glycolysis, transcription, and protein processing is not affected in kcs1DELTA. Deletion of OPI1, the INO1 transcription repressor, does not fully rescue INO1 expression in kcs1DELTA. Decreased inositol biosynthesis in kcs1DELTA is due to downregulation of INO1 transcription, but decreased inositol biosynthesis in kcs1DELTA is not because of perturbation of the UASINO regulatory complex Opi1-Ino2-Ino4, overview |
Saccharomyces cerevisiae |