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Literature summary for 2.7.4.21 extracted from

  • Weaver, J.D.; Wang, H.; Shears, S.B.
    The kinetic properties of a human PPIP5K reveal that its kinase activities are protected against the consequences of a deteriorating cellular bioenergetic environment (2013), Biosci. Rep., 33, e00022.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
K248A the mutant shows a reduction in 1D-myo-inositol hexakisphosphate-stimulated ATPase activity of isofom PPIP5K2. The mutant shows a significant reduction in the rate of 1D-myo-inositol phosphate-independent ATPase activity of isofom PPIP5K2 Homo sapiens
R213A the mutant shows a reduction in 1D-myo-inositol hexakisphosphate-stimulated ATPase activity of isofom PPIP5K2 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information isoform PPIP5K2 is insensitive to physiological changes in either [AMP] or [ATP]/[ADP] ratios Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00039
-
 1D-myo-inositol hexakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 m MKCl, at 37°C Homo sapiens
0.037
-
 ATP isoform PPIP5K2, using 1D-myo-inositol hexakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O43314
-
-

Purification (Commentary)

Purification (Comment) Organism
GSTrap HP column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol hexakisphosphate
-
 Homo sapiens ADP + 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate
-
 r

Synonyms

Synonyms Comment Organism
diphosphoinositol pentakisphosphate kinase 2
-
 Homo sapiens
PPIP5K2 isoform Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 1D-myo-inositol hexakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.79
-
 ATP isoform PPIP5K2, using 1D-myo-inositol hexakisphosphate as cosubstrate, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens
76
-
 1D-myo-inositol hexakisphosphate isoform PPIP5K2, in 20 mM HEPES-NaOH pH 7.2, 50 mM KCl, at 37°C Homo sapiens