Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3,4,5,6-inositol pentakisphosphate | IC50 for inositol hexakisphosphate kinase activity is 0.022 mM, IC50 for ATP synthase activity is 0.0128 mM | Rattus norvegicus | |
diphosphoinositol pentakisphosphate | IC50 for inositol hexakisphosphate kinase activity is 0.0066 mM, IC50 for ATP synthase activity is 0.0018 mM | Rattus norvegicus | |
inositol 1,2,4,5,6-pentakisphosphate | IC50 for inositol hexakisphosphate kinase activity is 0.022 mM | Rattus norvegicus | |
Inositol 1,3,4,5-tetrakisphosphate | IC50 for inositol hexakisphosphate kinase activity is 0.061 mM, IC50 for ATP synthase activity is 0.0325 mM | Rattus norvegicus | |
Inositol 1,4,5-trisphosphate | IC50 for ATP synthase activity is 0.253 mM | Rattus norvegicus | |
Inositol hexakisphosphate | IC50 for inositol hexakisphosphate kinase activity is 0.0008 mM, IC50 for ATP synthase activity is 0.0018 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
Inositol hexakisphosphate | pH 6.8, 37°C | Rattus norvegicus | |
0.00197 | - |
5-diphospho-1D-myo-inositol (1,2,3,4,6)pentakisphosphate | pH 6.8, 37°C | Rattus norvegicus | |
1.35 | - |
ATP | pH 6.8, 37°C | Rattus norvegicus | |
1.57 | - |
ADP | pH 6.8, 37°C | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
1 * 54000, SDS-PAGE | Rattus norvegicus |
60000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol hexakisphosphate | Rattus norvegicus | enzyme is responsible for the biosynthesis of diphospho-myo-inositol pentakisphosphate. The enzyme also has a ATP synthase activity, implying that 5-diphospho-1D-myo-inositol pentakisphosphate functions as high-energy phosphate donor | ADP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
heart | weak activity | Rattus norvegicus | - |
kidney | weak | Rattus norvegicus | - |
liver | weak activity | Rattus norvegicus | - |
thymus | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.558 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, 20% glycerol, optimal stability | Rattus norvegicus |
4°C, 7 days, 50% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol hexakisphosphate | forward and reverse reaction are random bireactant systems | Rattus norvegicus | ADP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | - |
? | |
ATP + 1D-myo-inositol hexakisphosphate | forward and reverse reaction are random bireactant systems | Rattus norvegicus | ADP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | - |
r | |
ATP + 1D-myo-inositol hexakisphosphate | enzyme is responsible for the biosynthesis of diphospho-myo-inositol pentakisphosphate. The enzyme also has a ATP synthase activity, implying that 5-diphospho-1D-myo-inositol pentakisphosphate functions as high-energy phosphate donor | Rattus norvegicus | ADP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 54000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0018 | - |
IC50 for inositol hexakisphosphate kinase activity is 0.0008 mM, IC50 for ATP synthase activity is 0.0018 mM | Rattus norvegicus | Inositol hexakisphosphate | |
0.0018 | - |
IC50 for inositol hexakisphosphate kinase activity is 0.0066 mM, IC50 for ATP synthase activity is 0.0018 mM | Rattus norvegicus | diphosphoinositol pentakisphosphate | |
0.0128 | - |
IC50 for inositol hexakisphosphate kinase activity is 0.022 mM, IC50 for ATP synthase activity is 0.0128 mM | Rattus norvegicus | 1,3,4,5,6-inositol pentakisphosphate | |
0.022 | - |
IC50 for inositol hexakisphosphate kinase activity is 0.022 mM | Rattus norvegicus | inositol 1,2,4,5,6-pentakisphosphate | |
0.0325 | - |
IC50 for inositol hexakisphosphate kinase activity is 0.061 mM, IC50 for ATP synthase activity is 0.0325 mM | Rattus norvegicus | His3Cd-GST-protein | |
0.253 | - |
IC50 for ATP synthase activity is 0.253 mM | Rattus norvegicus | human skin elastin |