Literature summary for 2.7.4.2 extracted from

Pilloff, D.; Dabovic, K.; Romanowski, M.J.; Bonanno, J.B.; Doherty, M.; Burley, S.K.; Leyh, T.S.
The kinetic mechanism of phosphomevalonate kinase (2003), J. Biol. Chem., 278, 4510-4515.

Search for more literature for 2.7.4.2

Inhibitors

Inhibitors	Comment	Organism	Structure
5-diphosphomevalonate	-	Streptococcus pneumoniae
ADP	-	Streptococcus pneumoniae
AMP-PNP	competitive dead-end inhibition vs. ATP	Streptococcus pneumoniae
ATP	-	Streptococcus pneumoniae
mevalonate	competitive vs. phosphomevalonate, noncompetitive vs. ATP	Streptococcus pneumoniae
phosphomevalonate	-	Streptococcus pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0042	-	phosphomevalonate	pH 7.0, 25°C	Streptococcus pneumoniae
0.012	-	diphosphomevalonate	pH 7.0, 25°C	Streptococcus pneumoniae
0.074	-	ATP	pH 7.0, 25°C	Streptococcus pneumoniae
0.35	-	ADP	pH 7.0, 25°C	Streptococcus pneumoniae
0.38	-	ADPbetaS	pH 7.0, 25°C	Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + (R)-5-phosphomevalonate	Streptococcus pneumoniae	enzyme of mevalonate pathway	ADP + 5-diphosphomevalonate	-	r

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADPbetaS + 5-diphosphomevalonate	41fold slower kcat than with ADP	Streptococcus pneumoniae	ATPbetaS + 5-phosphomevalonate	-	r
ATP + (R)-5-phosphomevalonate	enzyme of mevalonate pathway	Streptococcus pneumoniae	ADP + 5-diphosphomevalonate	-	r
ATP + 5-phosphomevalonate	-	Streptococcus pneumoniae	ADP + 5-diphosphomevalonate	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.095	-	ADPbetaS	pH 7.0, 25°C	Streptococcus pneumoniae
3.4	-	ATP	pH 7.0, 25°C	Streptococcus pneumoniae
3.9	-	ADP	pH 7.0, 25°C	Streptococcus pneumoniae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0077	-	phosphomevalonate	pH 7.0, 25°C	Streptococcus pneumoniae
0.014	-	5-diphosphomevalonate	pH 7.0, 25°C	Streptococcus pneumoniae
0.137	-	ATP	pH 7.0, 25°C	Streptococcus pneumoniae
0.41	-	ADP	pH 7.0, 25°C	Streptococcus pneumoniae

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Release 2023.1 (January 2023)