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Literature summary for 2.7.4.16 extracted from

  • Nishino, H.
    Biogenesis of cocarboxylase in Escherichia coli. Partial purification and some properties of thiamine monophosphate kinase (1972), J. Biochem., 72, 1093-1100.
    View publication on PubMed

General Stability

General Stability Organism
glycerol stabilizes during purification Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
ADP product inhibition Escherichia coli
AMP
-
Escherichia coli
Cs+ antagonizes stimulation by K+ Escherichia coli
diphosphate
-
Escherichia coli
EDTA
-
Escherichia coli
Li+ antagonizes stimulation by K+ Escherichia coli
additional information no inhibition by phosphate, KCN, iodoacetic acid, arsenate or arsenite Escherichia coli
N-ethylmaleimide
-
Escherichia coli
Na+ antagonizes stimulation by K+ Escherichia coli
NaF weak Escherichia coli
oxythiamine
-
Escherichia coli
PCMB 2-mercaptoethanol reverses Escherichia coli
pyrithiamine
-
Escherichia coli
Pyrithiamine phosphate
-
Escherichia coli
thiamine
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
thiamine phosphate pH 7.5, 37°C Escherichia coli
0.27
-
ATP pH 7.5, 37°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ requirement, about 95% as effective as Mg2+ Escherichia coli
Fe2+ activation, about 10% as effective as Mg2+ Escherichia coli
K+ activation Escherichia coli
Mg2+ requirement Escherichia coli
Mn2+ activation, about 30% as effective as Mg2+ Escherichia coli
additional information no activation by Ba2+, Ca2+, Cu2+, Cs+, Li+, Na+ Escherichia coli
NH4+ activation Escherichia coli
Rb+ activation Escherichia coli
Zn2+ activation, about 30% as effective as Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + thiamine phosphate Escherichia coli
-
ADP + thiamine diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K12
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000197
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 3 days, t1/2: 10 days Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + thiamine phosphate poor substrate Escherichia coli ?
-
?
ATP + thiamine phosphate
-
Escherichia coli ADP + thiamine diphosphate
-
?
ATP + thiamine phosphate no substrates: thiamine, GTP, CTP, UTP, AMP Escherichia coli ADP + thiamine diphosphate
-
?
ITP + thiamine phosphate poor substrate Escherichia coli ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.36
-
pyrithiamine pH 7.5, 37°C Escherichia coli