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Literature summary for 2.7.4.13 extracted from

  • Mikoulinskaia, G.V.; Zimin, A.A.; Feofanov, S.A.; Miroshnikov, A.I.
    Identification, cloning, and expression of bacteriophage T5 dnk gene encoding a broad specificity deoxyribonucleoside monophosphate kinase (EC 2.7.4.13) (2004), Protein Expr. Purif., 33, 166-175.
    View publication on PubMed
Search for more literature for 2.7.4.13

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Tequintavirus T5

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28670
-
 calculated from nucleotid sequence Tequintavirus T5
29140
-
 SDS-PAGE Tequintavirus T5

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deoxynucleoside monophosphate Tequintavirus T5 key step in de novo DNA precursor biosynthesis ADP + deoxynucleoside diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Tequintavirus T5 Q6QGP4
-
-

Purification (Commentary)

Purification (Comment) Organism
two alternative methods, the first involves ion-exchange chromatography on Mono Q, the second involves affinity chromatography on Toyopearl AF-Red, the protein is purified 5.34fold in both cases Tequintavirus T5

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29
-
 recombinant protein in sonicated cell exctracts, with dGMP as substrate Tequintavirus T5
36
-
 recombinant protein after Toyopearl 650 M flow-through, with dGMP as substrate Tequintavirus T5
40
-
 recombinant protein after ammonium sulphate precipitation, with dGMP as substrate Tequintavirus T5
84.2
-
 homogeneous recombinant enzyme with dCMP as substrate Tequintavirus T5
153.6
-
 homogeneous recombinant enzyme with dGMP as substrate Tequintavirus T5
155
-
 affinity chromatography eluant of the recombinant protein, with dGMP as substrate Tequintavirus T5
155
-
 ion-exchange chromatography MonoQ eluant of the recombinant protein, with dGMP as substrate Tequintavirus T5
166.8
-
 homogeneous recombinant enzyme with dTMP as substrate Tequintavirus T5
232
-
 homogeneous recombinant enzyme with dAMP as substrate Tequintavirus T5

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dAMP
-
 Tequintavirus T5 ADP + dADP
-
 ?
ATP + dCMP
-
 Tequintavirus T5 ADP + dCDP
-
 ?
ATP + deoxynucleoside monophosphate key step in de novo DNA precursor biosynthesis Tequintavirus T5 ADP + deoxynucleoside diphosphate
-
 ?
ATP + dGMP
-
 Tequintavirus T5 ADP + dGDP
-
 ?
ATP + dTMP
-
 Tequintavirus T5 ADP + dTDP
-
 ?

Synonyms

Synonyms Comment Organism
deoxyribonucleoside monophosphate kinase
-
 Tequintavirus T5
dNMP kinase
-
 Tequintavirus T5