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Literature summary for 2.7.4.13 extracted from
- Cloning of deoxynucleoside monophosphate kinase genes and biosynthesis of deoxynucleoside diphosphates (2006), Biotechnol. Bioeng., 93, 572-580.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain BL21 (DE3) | Saccharomyces cerevisiae |
| expression in Escherichia coli strain BL21 (DE3), CK expression at 37°C forms insoluble inclusion bodoes, therefore expression was performed at 25°C | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
ATP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae |  |
| 0.28 | - |
ATP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
| 0.33 | - |
dGMP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
| 0.48 | - |
ATP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
| 0.53 | - |
dCMP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
| 0.67 | - |
ATP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
| 0.89 | - |
dTMP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
| 1.1 | - |
dAMP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dAMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dAMP to the corresponding active sites | Saccharomyces cerevisiae | |
| Mg2+ | dCMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dCMP to the corresponding active sites | Saccharomyces cerevisiae | |
| Mg2+ | dGMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dGMP to the corresponding active sites | Saccharomyces cerevisiae | |
| Mg2+ | dTMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dTMP to the corresponding active sites | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20640 | - |
calculated from amino acid sequence | Saccharomyces cerevisiae |
| 22930 | - |
calculated from amino acid sequence | Saccharomyces cerevisiae |
| 24260 | - |
calculated from amino acid sequence | Saccharomyces cerevisiae |
| 24690 | - |
calculated from amino acid sequence | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + deoxynucleoside monophosphate | Saccharomyces cerevisiae | key step in de novo DNA precursor biosynthesis | ADP + deoxynucleoside diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
ATCC 2610 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Saccharomyces cerevisiae |
| 30 | - |
- |
Saccharomyces cerevisiae |
| 80 | - |
- |
Saccharomyces cerevisiae |
| 100 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dAMP | - |
Saccharomyces cerevisiae | ADP + dADP | - |
r | |
| ATP + dCMP | - |
Saccharomyces cerevisiae | ADP + dCDP | - |
r | |
| ATP + deoxynucleoside monophosphate | key step in de novo DNA precursor biosynthesis | Saccharomyces cerevisiae | ADP + deoxynucleoside diphosphate | - |
r | |
| ATP + dGMP | - |
Saccharomyces cerevisiae | ADP + dGDP | - |
r | |
| ATP + dTMP | - |
Saccharomyces cerevisiae | ADP + dTDP | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AK | - |
Saccharomyces cerevisiae |
| CK | - |
Saccharomyces cerevisiae |
| deoxyadenylate monophosphate kinase | - |
Saccharomyces cerevisiae |
| deoxycytidylate monophosphate kinase | - |
Saccharomyces cerevisiae |
| deoxyguanylate monophosphate kinase | - |
Saccharomyces cerevisiae |
| deoxynucleoside monophosphate kinase | - |
Saccharomyces cerevisiae |
| deoxythymidylate monophosphate kinase | - |
Saccharomyces cerevisiae |
| dNMP kinase | - |
Saccharomyces cerevisiae |
| GK | - |
Saccharomyces cerevisiae |
| TK | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
between pH 7-8 | Saccharomyces cerevisiae |
| 8 | - |
- |
Saccharomyces cerevisiae |
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