BRENDA - Enzyme Database show
show all sequences of 2.7.4.13

Cloning of deoxynucleoside monophosphate kinase genes and biosynthesis of deoxynucleoside diphosphates

Bao, J.; Ryu, D.D.; Biotechnol. Bioeng. 93, 572-580 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli strain BL21 (DE3); expression in Escherichia coli strain BL21 (DE3), CK expression at 37°C forms insoluble inclusion bodoes, therefore expression was performed at 25°C
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
0.28
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
0.33
-
dGMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
0.48
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
0.53
-
dCMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
0.67
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
0.89
-
dTMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
1.1
-
dAMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
dAMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dAMP to the corresponding active sites; dCMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dCMP to the corresponding active sites; dGMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dGMP to the corresponding active sites; dTMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dTMP to the corresponding active sites
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20640
-
calculated from amino acid sequence
Saccharomyces cerevisiae
22930
-
calculated from amino acid sequence
Saccharomyces cerevisiae
24260
-
calculated from amino acid sequence
Saccharomyces cerevisiae
24690
-
calculated from amino acid sequence
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + deoxynucleoside monophosphate
Saccharomyces cerevisiae
key step in de novo DNA precursor biosynthesis
ADP + deoxynucleoside diphosphate
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
ATCC 2610
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
20
-
-
Saccharomyces cerevisiae
30
-
-
Saccharomyces cerevisiae
80
-
-
Saccharomyces cerevisiae
100
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + dAMP
-
661371
Saccharomyces cerevisiae
ADP + dADP
-
-
-
r
ATP + dCMP
-
661371
Saccharomyces cerevisiae
ADP + dCDP
-
-
-
r
ATP + deoxynucleoside monophosphate
key step in de novo DNA precursor biosynthesis
661371
Saccharomyces cerevisiae
ADP + deoxynucleoside diphosphate
-
-
-
r
ATP + dGMP
-
661371
Saccharomyces cerevisiae
ADP + dGDP
-
-
-
r
ATP + dTMP
-
661371
Saccharomyces cerevisiae
ADP + dTDP
-
-
-
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
between pH 7-8
Saccharomyces cerevisiae
8
-
-
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli strain BL21 (DE3); expression in Escherichia coli strain BL21 (DE3), CK expression at 37°C forms insoluble inclusion bodoes, therefore expression was performed at 25°C
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
0.28
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
0.33
-
dGMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
0.48
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
0.53
-
dCMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
0.67
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
0.89
-
dTMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
1.1
-
dAMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
dAMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dAMP to the corresponding active sites; dCMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dCMP to the corresponding active sites; dGMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dGMP to the corresponding active sites; dTMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dTMP to the corresponding active sites
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20640
-
calculated from amino acid sequence
Saccharomyces cerevisiae
22930
-
calculated from amino acid sequence
Saccharomyces cerevisiae
24260
-
calculated from amino acid sequence
Saccharomyces cerevisiae
24690
-
calculated from amino acid sequence
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + deoxynucleoside monophosphate
Saccharomyces cerevisiae
key step in de novo DNA precursor biosynthesis
ADP + deoxynucleoside diphosphate
-
-
r
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
20
-
-
Saccharomyces cerevisiae
30
-
-
Saccharomyces cerevisiae
80
-
-
Saccharomyces cerevisiae
100
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + dAMP
-
661371
Saccharomyces cerevisiae
ADP + dADP
-
-
-
r
ATP + dCMP
-
661371
Saccharomyces cerevisiae
ADP + dCDP
-
-
-
r
ATP + deoxynucleoside monophosphate
key step in de novo DNA precursor biosynthesis
661371
Saccharomyces cerevisiae
ADP + deoxynucleoside diphosphate
-
-
-
r
ATP + dGMP
-
661371
Saccharomyces cerevisiae
ADP + dGDP
-
-
-
r
ATP + dTMP
-
661371
Saccharomyces cerevisiae
ADP + dTDP
-
-
-
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
between pH 7-8
Saccharomyces cerevisiae
8
-
-
Saccharomyces cerevisiae
Other publictions for EC 2.7.4.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739645
Mikoulinskaia
-
The study of the bacteriophage ...
Escherichia virus T5
Russ. J. Bioorg. Chem.
39
607-618
2013
-
-
1
-
15
-
-
-
-
1
-
4
-
1
-
-
1
-
-
-
5
-
9
1
1
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
15
-
-
-
-
-
-
1
-
4
-
-
-
1
-
-
5
-
9
1
1
-
-
-
2
-
-
-
-
1
1
-
-
-
661371
Bao
Cloning of deoxynucleoside mon ...
Saccharomyces cerevisiae
Biotechnol. Bioeng.
93
572-580
2006
-
-
1
-
-
-
-
8
-
1
4
1
-
3
-
-
-
-
-
-
4
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
8
-
1
4
1
-
-
-
-
-
-
4
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
663294
Mikoulinskaia
Identification, cloning, and e ...
Escherichia virus T5
Protein Expr. Purif.
33
166-175
2004
-
-
1
-
-
-
-
-
-
-
2
1
-
3
-
-
1
-
-
-
8
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
8
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642544
Mikoulinskaia
Purification and characterizat ...
Escherichia virus T5, no activity in Escherichia coli
Protein Expr. Purif.
27
195-201
2003
-
-
-
-
-
-
-
-
-
-
2
1
-
4
-
-
1
-
-
-
2
1
5
1
-
-
4
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
2
1
5
1
-
-
4
-
1
1
-
1
-
-
-
-
-
-
209854
Allen
T4 phage deoxyribonucleotide-s ...
Escherichia virus T4, no activity in Escherichia coli
J. Biol. Chem.
258
5746-5753
1983
-
-
-
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642542
Bessman
The enzymology of virus-infect ...
Escherichia virus T5, no activity in Escherichia coli
J. Biol. Chem.
240
439-445
1965
-
-
-
-
-
-
3
6
-
5
-
1
-
2
-
-
1
-
-
-
1
2
12
-
1
-
-
-
2
3
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
6
-
5
-
1
-
-
-
1
-
-
1
2
12
-
1
-
-
-
2
3
-
-
-
-
-
-
-
-