Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21 (DE3) | Saccharomyces cerevisiae |
expression in Escherichia coli strain BL21 (DE3), CK expression at 37°C forms insoluble inclusion bodoes, therefore expression was performed at 25°C | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
ATP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
0.28 | - |
ATP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
0.33 | - |
dGMP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
0.48 | - |
ATP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
0.53 | - |
dCMP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
0.67 | - |
ATP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
0.89 | - |
dTMP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae | |
1.1 | - |
dAMP | at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dAMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dAMP to the corresponding active sites | Saccharomyces cerevisiae | |
Mg2+ | dCMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dCMP to the corresponding active sites | Saccharomyces cerevisiae | |
Mg2+ | dGMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dGMP to the corresponding active sites | Saccharomyces cerevisiae | |
Mg2+ | dTMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dTMP to the corresponding active sites | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20640 | - |
calculated from amino acid sequence | Saccharomyces cerevisiae |
22930 | - |
calculated from amino acid sequence | Saccharomyces cerevisiae |
24260 | - |
calculated from amino acid sequence | Saccharomyces cerevisiae |
24690 | - |
calculated from amino acid sequence | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deoxynucleoside monophosphate | Saccharomyces cerevisiae | key step in de novo DNA precursor biosynthesis | ADP + deoxynucleoside diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
ATCC 2610 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Saccharomyces cerevisiae |
30 | - |
- |
Saccharomyces cerevisiae |
80 | - |
- |
Saccharomyces cerevisiae |
100 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dAMP | - |
Saccharomyces cerevisiae | ADP + dADP | - |
r | |
ATP + dCMP | - |
Saccharomyces cerevisiae | ADP + dCDP | - |
r | |
ATP + deoxynucleoside monophosphate | key step in de novo DNA precursor biosynthesis | Saccharomyces cerevisiae | ADP + deoxynucleoside diphosphate | - |
r | |
ATP + dGMP | - |
Saccharomyces cerevisiae | ADP + dGDP | - |
r | |
ATP + dTMP | - |
Saccharomyces cerevisiae | ADP + dTDP | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AK | - |
Saccharomyces cerevisiae |
CK | - |
Saccharomyces cerevisiae |
deoxyadenylate monophosphate kinase | - |
Saccharomyces cerevisiae |
deoxycytidylate monophosphate kinase | - |
Saccharomyces cerevisiae |
deoxyguanylate monophosphate kinase | - |
Saccharomyces cerevisiae |
deoxynucleoside monophosphate kinase | - |
Saccharomyces cerevisiae |
deoxythymidylate monophosphate kinase | - |
Saccharomyces cerevisiae |
dNMP kinase | - |
Saccharomyces cerevisiae |
GK | - |
Saccharomyces cerevisiae |
TK | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
between pH 7-8 | Saccharomyces cerevisiae |
8 | - |
- |
Saccharomyces cerevisiae |