BRENDA - Enzyme Database show
show all sequences of 2.7.4.13

Purification and characterization of the deoxynucleoside monophosphate kinase of bacteriophage T5

Mikoulinskaia, G.V.; Gubanov, S.I.; Zimin, A.A.; Kolesnikov, I.V.; Feofanov, S.A.; Miroshnikov, A.I.; Protein Expr. Purif. 27, 195-201 (2003)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
1 * 29000, SDS-PAGE
Escherichia virus T5
29140
-
analytical equilibrium ultracentrifugation
Escherichia virus T5
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + deoxynucleoside phosphate
Escherichia virus T5
enzyme induced during viral infection
ADP + deoxynucleoside diphosphate
-
Escherichia virus T5
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia virus T5
-
from infected Escherichia coli cells; mutant T5amH128a
-
no activity in Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
1190fold to homogeneity
Escherichia virus T5
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
166.8
-
purified enzyme, substrate dTMP
Escherichia virus T5
232
-
purified enzyme, substrate dAMP
Escherichia virus T5
Storage Stability
Storage Stability
Organism
-20°C, purified enzyme, 15% glycerol, only loss of a small part of activity after 6 months
Escherichia virus T5
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + dAMP
dAMP is the best acceptor substrate
642544
Escherichia virus T5
ADP + dADP
-
642544
Escherichia virus T5
r
ATP + dCMP
phosphorylated at 36% the rate of dAMP
642544
Escherichia virus T5
ADP + dCDP
-
-
-
r
ATP + deoxynucleoside phosphate
enzyme induced during viral infection
642544
Escherichia virus T5
ADP + deoxynucleoside diphosphate
-
642544
Escherichia virus T5
r
ATP + dGMP
phosphorylated at 65% the rate of dAMP
642544
Escherichia virus T5
ADP + dGDP
-
-
-
r
ATP + dTMP
phosphorylated at 70% the rate of dAMP
642544
Escherichia virus T5
ADP + dTDP
-
642544
Escherichia virus T5
r
Subunits
Subunits
Commentary
Organism
monomer
1 * 29000, SDS-PAGE
Escherichia virus T5
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
60 min, stable
Escherichia virus T5
42
-
10% loss of activity after 30 min
Escherichia virus T5
47
-
50% loss of activity after 30 min
Escherichia virus T5
52
-
rapid inactivation, t1/2: 5 min
Escherichia virus T5
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Escherichia virus T5
pH Range
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
active over a broad range
Escherichia virus T5
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Escherichia virus T5
determined under partially denaturing condiions
-
4.2
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
1 * 29000, SDS-PAGE
Escherichia virus T5
29140
-
analytical equilibrium ultracentrifugation
Escherichia virus T5
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + deoxynucleoside phosphate
Escherichia virus T5
enzyme induced during viral infection
ADP + deoxynucleoside diphosphate
-
Escherichia virus T5
r
Purification (Commentary) (protein specific)
Commentary
Organism
1190fold to homogeneity
Escherichia virus T5
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
166.8
-
purified enzyme, substrate dTMP
Escherichia virus T5
232
-
purified enzyme, substrate dAMP
Escherichia virus T5
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, purified enzyme, 15% glycerol, only loss of a small part of activity after 6 months
Escherichia virus T5
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + dAMP
dAMP is the best acceptor substrate
642544
Escherichia virus T5
ADP + dADP
-
642544
Escherichia virus T5
r
ATP + dCMP
phosphorylated at 36% the rate of dAMP
642544
Escherichia virus T5
ADP + dCDP
-
-
-
r
ATP + deoxynucleoside phosphate
enzyme induced during viral infection
642544
Escherichia virus T5
ADP + deoxynucleoside diphosphate
-
642544
Escherichia virus T5
r
ATP + dGMP
phosphorylated at 65% the rate of dAMP
642544
Escherichia virus T5
ADP + dGDP
-
-
-
r
ATP + dTMP
phosphorylated at 70% the rate of dAMP
642544
Escherichia virus T5
ADP + dTDP
-
642544
Escherichia virus T5
r
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 29000, SDS-PAGE
Escherichia virus T5
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
60 min, stable
Escherichia virus T5
42
-
10% loss of activity after 30 min
Escherichia virus T5
47
-
50% loss of activity after 30 min
Escherichia virus T5
52
-
rapid inactivation, t1/2: 5 min
Escherichia virus T5
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Escherichia virus T5
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
active over a broad range
Escherichia virus T5
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Escherichia virus T5
determined under partially denaturing condiions
-
4.2
Other publictions for EC 2.7.4.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739645
Mikoulinskaia
-
The study of the bacteriophage ...
Escherichia virus T5
Russ. J. Bioorg. Chem.
39
607-618
2013
-
-
1
-
15
-
-
-
-
1
-
4
-
1
-
-
1
-
-
-
5
-
9
1
1
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
15
-
-
-
-
-
-
1
-
4
-
-
-
1
-
-
5
-
9
1
1
-
-
-
2
-
-
-
-
1
1
-
-
-
661371
Bao
Cloning of deoxynucleoside mon ...
Saccharomyces cerevisiae
Biotechnol. Bioeng.
93
572-580
2006
-
-
1
-
-
-
-
8
-
1
4
1
-
3
-
-
-
-
-
-
4
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
8
-
1
4
1
-
-
-
-
-
-
4
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
663294
Mikoulinskaia
Identification, cloning, and e ...
Escherichia virus T5
Protein Expr. Purif.
33
166-175
2004
-
-
1
-
-
-
-
-
-
-
2
1
-
3
-
-
1
-
-
-
8
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
8
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642544
Mikoulinskaia
Purification and characterizat ...
Escherichia virus T5, no activity in Escherichia coli
Protein Expr. Purif.
27
195-201
2003
-
-
-
-
-
-
-
-
-
-
2
1
-
4
-
-
1
-
-
-
2
1
5
1
-
-
4
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
2
1
5
1
-
-
4
-
1
1
-
1
-
-
-
-
-
-
209854
Allen
T4 phage deoxyribonucleotide-s ...
Escherichia virus T4, no activity in Escherichia coli
J. Biol. Chem.
258
5746-5753
1983
-
-
-
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642542
Bessman
The enzymology of virus-infect ...
Escherichia virus T5, no activity in Escherichia coli
J. Biol. Chem.
240
439-445
1965
-
-
-
-
-
-
3
6
-
5
-
1
-
2
-
-
1
-
-
-
1
2
12
-
1
-
-
-
2
3
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
6
-
5
-
1
-
-
-
1
-
-
1
2
12
-
1
-
-
-
2
3
-
-
-
-
-
-
-
-