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Literature summary for 2.7.4.13 extracted from

  • Mikoulinskaia, G.V.; Gubanov, S.I.; Zimin, A.A.; Kolesnikov, I.V.; Feofanov, S.A.; Miroshnikov, A.I.
    Purification and characterization of the deoxynucleoside monophosphate kinase of bacteriophage T5 (2003), Protein Expr. Purif., 27, 195-201.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
1 * 29000, SDS-PAGE Tequintavirus T5
29140
-
analytical equilibrium ultracentrifugation Tequintavirus T5

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deoxynucleoside phosphate Tequintavirus T5 enzyme induced during viral infection ADP + deoxynucleoside diphosphate
-
r

Organism

Organism UniProt Comment Textmining
no activity in Escherichia coli
-
-
-
Tequintavirus T5
-
from infected Escherichia coli cells
-
Tequintavirus T5
-
mutant T5amH128a
-

Purification (Commentary)

Purification (Comment) Organism
1190fold to homogeneity Tequintavirus T5

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
166.8
-
purified enzyme, substrate dTMP Tequintavirus T5
232
-
purified enzyme, substrate dAMP Tequintavirus T5

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 15% glycerol, only loss of a small part of activity after 6 months Tequintavirus T5

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dAMP dAMP is the best acceptor substrate Tequintavirus T5 ADP + dADP
-
r
ATP + dCMP phosphorylated at 36% the rate of dAMP Tequintavirus T5 ADP + dCDP
-
r
ATP + deoxynucleoside phosphate enzyme induced during viral infection Tequintavirus T5 ADP + deoxynucleoside diphosphate
-
r
ATP + dGMP phosphorylated at 65% the rate of dAMP Tequintavirus T5 ADP + dGDP
-
r
ATP + dTMP phosphorylated at 70% the rate of dAMP Tequintavirus T5 ADP + dTDP
-
r

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS-PAGE Tequintavirus T5

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
60 min, stable Tequintavirus T5
42
-
10% loss of activity after 30 min Tequintavirus T5
47
-
50% loss of activity after 30 min Tequintavirus T5
52
-
rapid inactivation, t1/2: 5 min Tequintavirus T5

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Tequintavirus T5

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
active over a broad range Tequintavirus T5

pI Value

Organism Comment pI Value Maximum pI Value
Tequintavirus T5 determined under partially denaturing condiions
-
4.2