Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dAMP | competitive, dTMP as substrate | Tequintavirus T5 | |
dCMP | competitive, dTMP as substrate | Tequintavirus T5 | |
dGMP | competitive, dTMP as substrate | Tequintavirus T5 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
dCMP | pH 7.5, 37°C | Tequintavirus T5 | |
0.173 | - |
dTMP | pH 7.5, 37°C | Tequintavirus T5 | |
0.22 | - |
dAMP | pH 7.5, 37°C | Tequintavirus T5 | |
0.22 | - |
dGMP | pH 7.5, 37°C | Tequintavirus T5 | |
0.36 | - |
ATP | pH 7.5, 37°C | Tequintavirus T5 | |
2.8 | - |
dUMP | pH 7.5, 37°C | Tequintavirus T5 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | requirement, can replace Mg2+ | Tequintavirus T5 | |
Fe2+ | activation, less effective than Mg2+, Co2+, Mn2+ | Tequintavirus T5 | |
Mg2+ | requirement, can replace Co2+ | Tequintavirus T5 | |
Mn2+ | requirement, can replace Co2+ or Mg2+ | Tequintavirus T5 | |
additional information | no activation by Ba2+, Ca2+, Cd2+, Cr3+, Cu2+, Fe3+, Hg2+, Ni2+, Zn2+ | Tequintavirus T5 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deoxynucleoside phosphate | Tequintavirus T5 | enzyme induced during viral infection | ADP + deoxynucleoside diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Escherichia coli | - |
- |
- |
Tequintavirus T5 | - |
from infected Escherichia coli cells | - |
Purification (Comment) | Organism |
---|---|
500fold | Tequintavirus T5 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.7 | - |
purified enzyme | Tequintavirus T5 |
Storage Stability | Organism |
---|---|
0°C, 25% loss of activity per day in crude cell extract | Tequintavirus T5 |
4°C, several months, ionic strength 0.1 or above | Tequintavirus T5 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-bromo-dUMP | phosphorylated at 45% the rate of dAMP | Tequintavirus T5 | ADP + 5-bromo-dUDP | - |
r | |
ATP + dAMP | dAMP is the best acceptor substrate | Tequintavirus T5 | ADP + dADP | - |
r | |
ATP + dAMP | dATP can replace ATP with less efficiency | Tequintavirus T5 | ADP + dADP | - |
r | |
ATP + dCMP | phosphorylated at 30% the rate of dAMP, dATP can replace ATP | Tequintavirus T5 | ADP + dCDP | - |
r | |
ATP + deoxynucleoside phosphate | enzyme induced during viral infection | Tequintavirus T5 | ADP + deoxynucleoside diphosphate | - |
r | |
ATP + dGMP | dATP can replace ATP | Tequintavirus T5 | ADP + dGDP | - |
r | |
ATP + dGMP | phosphorylated at 65% the rate of dAMP | Tequintavirus T5 | ADP + dGDP | - |
r | |
ATP + dTMP | dATP can replace ATP with less efficiency | Tequintavirus T5 | ADP + dTDP | - |
r | |
ATP + dTMP | phosphorylated at 70% the rate of dAMP | Tequintavirus T5 | ADP + dTDP | - |
r | |
ATP + dUMP | - |
Tequintavirus T5 | ADP + dUDP | - |
? | |
additional information | very poor acceptor substrates are AMP, CMP or dUMP | Tequintavirus T5 | ? | - |
? | |
additional information | no acceptor substrates are dIMP, deoxyxanthylate, 5-hydroxymethyldeoxycytidylate, UMP, GMP, 5-bromo-dUMP, 5-methyl-dCMP or deoxythymidine | Tequintavirus T5 | ? | - |
? | |
additional information | no donor substrates are UTP, GTP, CTP, dTTP, dGTP or dCTP | Tequintavirus T5 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Tequintavirus T5 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Tequintavirus T5 |
7.6 | - |
substrate dTMP | Tequintavirus T5 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 9 | about 80% of maximal activity at pH 6.2 and about 65% of maximal activity at pH 9 with substrate dGMP | Tequintavirus T5 |
6.2 | 9.4 | about 65% of maximal activity at pH 6.2 and about 70% of maximal activity at pH 9.4 with substrate dAMP | Tequintavirus T5 |
6.3 | 9 | at pH 6.3: about 70% of maximal activity with substrate dTMP, about 85% of maximal activity with substrate dCMP, at pH 9.0: about 50% of maximal activity with substrate dTMP, 65% of maximal activity with substrate dCMP | Tequintavirus T5 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
dCMP | dTMP as substrate, pH 7.6, 37°C | Tequintavirus T5 | |
0.15 | - |
dAMP | dTMP as substrate, pH 7.6, 37°C | Tequintavirus T5 | |
0.15 | - |
dGMP | dTMP as substrate, pH 7.6, 37°C | Tequintavirus T5 |