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Literature summary for 2.7.4.13 extracted from

  • Bessman, M.J.; Herriott, S.T.; Van Bibber Orr, M.J.
    The enzymology of virus-infected bacteria. VI. Purification and properties of the deoxynucleotide kinase induced by bacteriophage T5 (1965), J. Biol. Chem., 240, 439-445.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
dAMP competitive, dTMP as substrate Tequintavirus T5
dCMP competitive, dTMP as substrate Tequintavirus T5
dGMP competitive, dTMP as substrate Tequintavirus T5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
dCMP pH 7.5, 37°C Tequintavirus T5
0.173
-
dTMP pH 7.5, 37°C Tequintavirus T5
0.22
-
dAMP pH 7.5, 37°C Tequintavirus T5
0.22
-
dGMP pH 7.5, 37°C Tequintavirus T5
0.36
-
ATP pH 7.5, 37°C Tequintavirus T5
2.8
-
dUMP pH 7.5, 37°C Tequintavirus T5

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ requirement, can replace Mg2+ Tequintavirus T5
Fe2+ activation, less effective than Mg2+, Co2+, Mn2+ Tequintavirus T5
Mg2+ requirement, can replace Co2+ Tequintavirus T5
Mn2+ requirement, can replace Co2+ or Mg2+ Tequintavirus T5
additional information no activation by Ba2+, Ca2+, Cd2+, Cr3+, Cu2+, Fe3+, Hg2+, Ni2+, Zn2+ Tequintavirus T5

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deoxynucleoside phosphate Tequintavirus T5 enzyme induced during viral infection ADP + deoxynucleoside diphosphate
-
r

Organism

Organism UniProt Comment Textmining
no activity in Escherichia coli
-
-
-
Tequintavirus T5
-
from infected Escherichia coli cells
-

Purification (Commentary)

Purification (Comment) Organism
500fold Tequintavirus T5

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.7
-
purified enzyme Tequintavirus T5

Storage Stability

Storage Stability Organism
0°C, 25% loss of activity per day in crude cell extract Tequintavirus T5
4°C, several months, ionic strength 0.1 or above Tequintavirus T5

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-bromo-dUMP phosphorylated at 45% the rate of dAMP Tequintavirus T5 ADP + 5-bromo-dUDP
-
r
ATP + dAMP dAMP is the best acceptor substrate Tequintavirus T5 ADP + dADP
-
r
ATP + dAMP dATP can replace ATP with less efficiency Tequintavirus T5 ADP + dADP
-
r
ATP + dCMP phosphorylated at 30% the rate of dAMP, dATP can replace ATP Tequintavirus T5 ADP + dCDP
-
r
ATP + deoxynucleoside phosphate enzyme induced during viral infection Tequintavirus T5 ADP + deoxynucleoside diphosphate
-
r
ATP + dGMP dATP can replace ATP Tequintavirus T5 ADP + dGDP
-
r
ATP + dGMP phosphorylated at 65% the rate of dAMP Tequintavirus T5 ADP + dGDP
-
r
ATP + dTMP dATP can replace ATP with less efficiency Tequintavirus T5 ADP + dTDP
-
r
ATP + dTMP phosphorylated at 70% the rate of dAMP Tequintavirus T5 ADP + dTDP
-
r
ATP + dUMP
-
Tequintavirus T5 ADP + dUDP
-
?
additional information very poor acceptor substrates are AMP, CMP or dUMP Tequintavirus T5 ?
-
?
additional information no acceptor substrates are dIMP, deoxyxanthylate, 5-hydroxymethyldeoxycytidylate, UMP, GMP, 5-bromo-dUMP, 5-methyl-dCMP or deoxythymidine Tequintavirus T5 ?
-
?
additional information no donor substrates are UTP, GTP, CTP, dTTP, dGTP or dCTP Tequintavirus T5 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Tequintavirus T5

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Tequintavirus T5
7.6
-
substrate dTMP Tequintavirus T5

pH Range

pH Minimum pH Maximum Comment Organism
6.2 9 about 80% of maximal activity at pH 6.2 and about 65% of maximal activity at pH 9 with substrate dGMP Tequintavirus T5
6.2 9.4 about 65% of maximal activity at pH 6.2 and about 70% of maximal activity at pH 9.4 with substrate dAMP Tequintavirus T5
6.3 9 at pH 6.3: about 70% of maximal activity with substrate dTMP, about 85% of maximal activity with substrate dCMP, at pH 9.0: about 50% of maximal activity with substrate dTMP, 65% of maximal activity with substrate dCMP Tequintavirus T5

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.032
-
dCMP dTMP as substrate, pH 7.6, 37°C Tequintavirus T5
0.15
-
dAMP dTMP as substrate, pH 7.6, 37°C Tequintavirus T5
0.15
-
dGMP dTMP as substrate, pH 7.6, 37°C Tequintavirus T5