Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-hydroxymethyl-dCMP | dGMP or dTMP as substrate | Escherichia coli | |
dGMP | dTMP or 5-hydroxymethyl-dCMP as substrate | Escherichia coli | |
dTMP | dGMP or 5-hydroxymethyl-dCMP as substrate | Escherichia coli | |
additional information | tryptic digestion inactivates | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
5-hydroxymethyl-dCMP | pH 7.4, 37°C | Escherichia coli | |
0.085 | - |
dGMP | pH 7.4, 37°C | Escherichia coli | |
0.278 | 0.313 | dTMP | pH 7.4, 37°C | Escherichia coli | |
0.82 | - |
ATP | pH 7.4, 37°C, with dTMP | Escherichia coli | |
1.25 | - |
ATP | pH 7.4, 37°C, with dGMP | Escherichia coli | |
5 | - |
ATP | pH 7.4, 37°C, with 5-hydroxymethyl-dCMP | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | requirement, about 60% as effective as Mn2+, dGMP as substrate | Escherichia coli | |
Mg2+ | requirement, about 90% as effective as Mn2+, dGMP as substrate | Escherichia coli | |
Mn2+ | requirement, dGMP as substrate | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dGMP | Escherichia coli | or dTMP | ADP + dGDP | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
infected with bacteriophage T2 | - |
Purification (Comment) | Organism |
---|---|
infected with bacteriophage T2 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.16 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-10°C, crude preparation, at least a year | Escherichia coli |
4°C, partially purified preparation, at least 2 months | Escherichia coli |
at -10°C to 4°C, 1 mM EDTA, up to 20% loss of activity within 6 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-hydroxymethyl-dCMP | phosphorylation at about half the rate of dGMP or dTMP | Escherichia coli | ADP + 5-hydroxymethyl-dCDP | - |
r | |
ATP + dGMP | as good as dTMP | Escherichia coli | ADP + dGDP | - |
r | |
ATP + dGMP | or dTMP | Escherichia coli | ADP + dGDP | - |
r | |
ATP + dTMP | as good as dGMP | Escherichia coli | ADP + dTDP | - |
r | |
dATP + dGMP | phosphorylation at about 80-90% the rate of ATP | Escherichia coli | dADP + dGDP | - |
r | |
dATP + dTMP | phosphorylation at about 80-90% the rate of ATP | Escherichia coli | dADP + dTDP | - |
r | |
additional information | poor substrates are dUMP or 5-methyl-dCMP, no donor substrates are GTP, CTP, dGTP, dCTP, or dTTP, no acceptor substrates are dCMP, dAMP, GMP, or UMP | Escherichia coli | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
dTMP or dGMP | Escherichia coli |
8.6 | - |
5-hydroxymethyl-dCMP | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.3 | pH 7: about 75% of maximal activity of 5-hydroxymethyl-dCMP, 84% of maximal activity dTMP or 87% of maximal activity of dGMP, pH 9.3: about 79% of maximal activity of dTMP, 83% of maximal activity of dGMP or 90% of maximal activity of 5-hydroxymethyl-dCMP | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
3 min, about 60% loss of activity at 37°C | Escherichia coli |
4.4 | - |
37°C, 3 min, about 30% loss of activity, and about 45% loss of activity within 6 min | Escherichia coli |
4.7 | - |
15 min, about 20% loss of activity at 37°C | Escherichia coli |
5.4 | - |
15 min stable at 37°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
5-hydroxymethyl-dCMP | pH 7.4, 37°C, dTMP is substrate | Escherichia coli | |
0.044 | - |
5-hydroxymethyl-dCMP | pH 7.4, 37°C, dGMP is substrate | Escherichia coli | |
0.064 | - |
dGMP | pH 7.4, 37°C, dTMP is substrate | Escherichia coli | |
0.25 | - |
dTMP | pH 7.4, 37°C, 5-hydroxymethyl-dCMP is substrate | Escherichia coli | |
0.314 | - |
dTMP | pH 7.4, 37°C, dGMP is substrate | Escherichia coli | |
1.28 | - |
dGMP | pH 7.4, 37°C, 5-hydroxymethyl-dCMP is substrate | Escherichia coli |