BRENDA - Enzyme Database
show all sequences of 2.7.4.12

The enzymology of virus-infected bacteria, IV. Purification and properties of the deoxynucleotide kinase induced by bacteriophage T2

Bello, L.J.; Bessman, M.J.; J. Biol. Chem. 238, 1777-1787 (1963)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5-hydroxymethyl-dCMP
dGMP or dTMP as substrate
Escherichia coli
dGMP
dTMP or 5-hydroxymethyl-dCMP as substrate
Escherichia coli
dTMP
dGMP or 5-hydroxymethyl-dCMP as substrate
Escherichia coli
additional information
tryptic digestion inactivates
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.056
-
5-hydroxymethyl-dCMP
pH 7.4, 37C
Escherichia coli
0.085
-
dGMP
pH 7.4, 37C
Escherichia coli
0.278
0.313
dTMP
pH 7.4, 37C
Escherichia coli
0.82
-
ATP
pH 7.4, 37C, with dTMP
Escherichia coli
1.25
-
ATP
pH 7.4, 37C, with dGMP
Escherichia coli
5
-
ATP
pH 7.4, 37C, with 5-hydroxymethyl-dCMP
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
requirement, about 60% as effective as Mn2+, dGMP as substrate
Escherichia coli
Mg2+
requirement, about 90% as effective as Mn2+, dGMP as substrate
Escherichia coli
Mn2+
requirement, dGMP as substrate
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + dGMP
Escherichia coli
or dTMP
ADP + dGDP
-
Escherichia coli
r
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
infected with bacteriophage T2
-
Purification (Commentary)
Purification (Commentary)
Organism
infected with bacteriophage T2
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
24.16
-
-
Escherichia coli
Storage Stability
Storage Stability
Organism
-10C, crude preparation, at least a year
Escherichia coli
4C, partially purified preparation, at least 2 months
Escherichia coli
at -10C to 4C, 1 mM EDTA, up to 20% loss of activity within 6 months
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + 5-hydroxymethyl-dCMP
phosphorylation at about half the rate of dGMP or dTMP
642541
Escherichia coli
ADP + 5-hydroxymethyl-dCDP
-
642541
Escherichia coli
r
ATP + dGMP
as good as dTMP
642541
Escherichia coli
ADP + dGDP
-
642541
Escherichia coli
r
ATP + dGMP
or dTMP
642541
Escherichia coli
ADP + dGDP
-
642541
Escherichia coli
r
ATP + dTMP
as good as dGMP
642541
Escherichia coli
ADP + dTDP
-
642541
Escherichia coli
r
dATP + dGMP
phosphorylation at about 80-90% the rate of ATP
642541
Escherichia coli
dADP + dGDP
-
-
-
r
dATP + dTMP
phosphorylation at about 80-90% the rate of ATP
642541
Escherichia coli
dADP + dTDP
-
-
-
r
additional information
poor substrates are dUMP or 5-methyl-dCMP, no donor substrates are GTP, CTP, dGTP, dCTP, or dTTP, no acceptor substrates are dCMP, dAMP, GMP, or UMP
642541
Escherichia coli
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
dTMP or dGMP
Escherichia coli
8.6
-
5-hydroxymethyl-dCMP
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9.3
pH 7: about 75% of maximal activity of 5-hydroxymethyl-dCMP, 84% of maximal activity dTMP or 87% of maximal activity of dGMP, pH 9.3: about 79% of maximal activity of dTMP, 83% of maximal activity of dGMP or 90% of maximal activity of 5-hydroxymethyl-dCMP
Escherichia coli
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
3 min, about 60% loss of activity at 37C
Escherichia coli
4.4
-
37C, 3 min, about 30% loss of activity, and about 45% loss of activity within 6 min
Escherichia coli
4.7
-
15 min, about 20% loss of activity at 37C
Escherichia coli
5.4
-
15 min stable at 37C
Escherichia coli
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.04
-
5-hydroxymethyl-dCMP
pH 7.4, 37C, dTMP is substrate
Escherichia coli
0.044
-
5-hydroxymethyl-dCMP
pH 7.4, 37C, dGMP is substrate
Escherichia coli
0.064
-
dGMP
pH 7.4, 37C, dTMP is substrate
Escherichia coli
0.25
-
dTMP
pH 7.4, 37C, 5-hydroxymethyl-dCMP is substrate
Escherichia coli
0.314
-
dTMP
pH 7.4, 37C, dGMP is substrate
Escherichia coli
1.28
-
dGMP
pH 7.4, 37C, 5-hydroxymethyl-dCMP is substrate
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5-hydroxymethyl-dCMP
dGMP or dTMP as substrate
Escherichia coli
dGMP
dTMP or 5-hydroxymethyl-dCMP as substrate
Escherichia coli
dTMP
dGMP or 5-hydroxymethyl-dCMP as substrate
Escherichia coli
additional information
tryptic digestion inactivates
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.04
-
5-hydroxymethyl-dCMP
pH 7.4, 37C, dTMP is substrate
Escherichia coli
0.044
-
5-hydroxymethyl-dCMP
pH 7.4, 37C, dGMP is substrate
Escherichia coli
0.064
-
dGMP
pH 7.4, 37C, dTMP is substrate
Escherichia coli
0.25
-
dTMP
pH 7.4, 37C, 5-hydroxymethyl-dCMP is substrate
Escherichia coli
0.314
-
dTMP
pH 7.4, 37C, dGMP is substrate
Escherichia coli
1.28
-
dGMP
pH 7.4, 37C, 5-hydroxymethyl-dCMP is substrate
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.056
-
5-hydroxymethyl-dCMP
pH 7.4, 37C
Escherichia coli
0.085
-
dGMP
pH 7.4, 37C
Escherichia coli
0.278
0.313
dTMP
pH 7.4, 37C
Escherichia coli
0.82
-
ATP
pH 7.4, 37C, with dTMP
Escherichia coli
1.25
-
ATP
pH 7.4, 37C, with dGMP
Escherichia coli
5
-
ATP
pH 7.4, 37C, with 5-hydroxymethyl-dCMP
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
requirement, about 60% as effective as Mn2+, dGMP as substrate
Escherichia coli
Mg2+
requirement, about 90% as effective as Mn2+, dGMP as substrate
Escherichia coli
Mn2+
requirement, dGMP as substrate
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + dGMP
Escherichia coli
or dTMP
ADP + dGDP
-
Escherichia coli
r
Purification (Commentary) (protein specific)
Commentary
Organism
infected with bacteriophage T2
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
24.16
-
-
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-10C, crude preparation, at least a year
Escherichia coli
4C, partially purified preparation, at least 2 months
Escherichia coli
at -10C to 4C, 1 mM EDTA, up to 20% loss of activity within 6 months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + 5-hydroxymethyl-dCMP
phosphorylation at about half the rate of dGMP or dTMP
642541
Escherichia coli
ADP + 5-hydroxymethyl-dCDP
-
642541
Escherichia coli
r
ATP + dGMP
as good as dTMP
642541
Escherichia coli
ADP + dGDP
-
642541
Escherichia coli
r
ATP + dGMP
or dTMP
642541
Escherichia coli
ADP + dGDP
-
642541
Escherichia coli
r
ATP + dTMP
as good as dGMP
642541
Escherichia coli
ADP + dTDP
-
642541
Escherichia coli
r
dATP + dGMP
phosphorylation at about 80-90% the rate of ATP
642541
Escherichia coli
dADP + dGDP
-
-
-
r
dATP + dTMP
phosphorylation at about 80-90% the rate of ATP
642541
Escherichia coli
dADP + dTDP
-
-
-
r
additional information
poor substrates are dUMP or 5-methyl-dCMP, no donor substrates are GTP, CTP, dGTP, dCTP, or dTTP, no acceptor substrates are dCMP, dAMP, GMP, or UMP
642541
Escherichia coli
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
dTMP or dGMP
Escherichia coli
8.6
-
5-hydroxymethyl-dCMP
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9.3
pH 7: about 75% of maximal activity of 5-hydroxymethyl-dCMP, 84% of maximal activity dTMP or 87% of maximal activity of dGMP, pH 9.3: about 79% of maximal activity of dTMP, 83% of maximal activity of dGMP or 90% of maximal activity of 5-hydroxymethyl-dCMP
Escherichia coli
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
3 min, about 60% loss of activity at 37C
Escherichia coli
4.4
-
37C, 3 min, about 30% loss of activity, and about 45% loss of activity within 6 min
Escherichia coli
4.7
-
15 min, about 20% loss of activity at 37C
Escherichia coli
5.4
-
15 min stable at 37C
Escherichia coli
Other publictions for EC 2.7.4.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
642541
Bello
The enzymology of virus-infect ...
Escherichia coli
J. Biol. Chem.
238
1777-1787
1963
-
-
-
-
-
-
4
6
-
3
-
1
-
4
-
-
1
-
-
-
1
3
7
-
-
1
-
-
-
2
1
4
-
6
-
-
-
-
-
-
-
-
-
-
4
6
6
-
3
-
1
-
-
-
1
-
-
1
3
7
-
1
-
-
-
2
1
4
-
-
-
-
-
-
-