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Literature summary for 2.7.4.12 extracted from

  • Bello, L.J.; Bessman, M.J.
    The enzymology of virus-infected bacteria, IV. Purification and properties of the deoxynucleotide kinase induced by bacteriophage T2 (1963), J. Biol. Chem., 238, 1777-1787.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5-hydroxymethyl-dCMP dGMP or dTMP as substrate Escherichia coli
dGMP dTMP or 5-hydroxymethyl-dCMP as substrate Escherichia coli
dTMP dGMP or 5-hydroxymethyl-dCMP as substrate Escherichia coli
additional information tryptic digestion inactivates Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
5-hydroxymethyl-dCMP pH 7.4, 37°C Escherichia coli
0.085
-
dGMP pH 7.4, 37°C Escherichia coli
0.278 0.313 dTMP pH 7.4, 37°C Escherichia coli
0.82
-
ATP pH 7.4, 37°C, with dTMP Escherichia coli
1.25
-
ATP pH 7.4, 37°C, with dGMP Escherichia coli
5
-
ATP pH 7.4, 37°C, with 5-hydroxymethyl-dCMP Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement, about 60% as effective as Mn2+, dGMP as substrate Escherichia coli
Mg2+ requirement, about 90% as effective as Mn2+, dGMP as substrate Escherichia coli
Mn2+ requirement, dGMP as substrate Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + dGMP Escherichia coli or dTMP ADP + dGDP
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
infected with bacteriophage T2
-

Purification (Commentary)

Purification (Comment) Organism
infected with bacteriophage T2 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.16
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-10°C, crude preparation, at least a year Escherichia coli
4°C, partially purified preparation, at least 2 months Escherichia coli
at -10°C to 4°C, 1 mM EDTA, up to 20% loss of activity within 6 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-hydroxymethyl-dCMP phosphorylation at about half the rate of dGMP or dTMP Escherichia coli ADP + 5-hydroxymethyl-dCDP
-
r
ATP + dGMP as good as dTMP Escherichia coli ADP + dGDP
-
r
ATP + dGMP or dTMP Escherichia coli ADP + dGDP
-
r
ATP + dTMP as good as dGMP Escherichia coli ADP + dTDP
-
r
dATP + dGMP phosphorylation at about 80-90% the rate of ATP Escherichia coli dADP + dGDP
-
r
dATP + dTMP phosphorylation at about 80-90% the rate of ATP Escherichia coli dADP + dTDP
-
r
additional information poor substrates are dUMP or 5-methyl-dCMP, no donor substrates are GTP, CTP, dGTP, dCTP, or dTTP, no acceptor substrates are dCMP, dAMP, GMP, or UMP Escherichia coli ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
dTMP or dGMP Escherichia coli
8.6
-
5-hydroxymethyl-dCMP Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7 9.3 pH 7: about 75% of maximal activity of 5-hydroxymethyl-dCMP, 84% of maximal activity dTMP or 87% of maximal activity of dGMP, pH 9.3: about 79% of maximal activity of dTMP, 83% of maximal activity of dGMP or 90% of maximal activity of 5-hydroxymethyl-dCMP Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
3 min, about 60% loss of activity at 37°C Escherichia coli
4.4
-
37°C, 3 min, about 30% loss of activity, and about 45% loss of activity within 6 min Escherichia coli
4.7
-
15 min, about 20% loss of activity at 37°C Escherichia coli
5.4
-
15 min stable at 37°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.04
-
5-hydroxymethyl-dCMP pH 7.4, 37°C, dTMP is substrate Escherichia coli
0.044
-
5-hydroxymethyl-dCMP pH 7.4, 37°C, dGMP is substrate Escherichia coli
0.064
-
dGMP pH 7.4, 37°C, dTMP is substrate Escherichia coli
0.25
-
dTMP pH 7.4, 37°C, 5-hydroxymethyl-dCMP is substrate Escherichia coli
0.314
-
dTMP pH 7.4, 37°C, dGMP is substrate Escherichia coli
1.28
-
dGMP pH 7.4, 37°C, 5-hydroxymethyl-dCMP is substrate Escherichia coli