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Literature summary for 2.7.4.11 extracted from
- The partial purification of deoxynucleoside monophosphate kinases from L cells (1965), Biochim. Biophys. Acta, 108, 114-124.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | substrate inhibition | Mus musculus |  |
| EDTA | - |
Mus musculus | |
| additional information | no inhibition by p-hydroxymercuribenzoate | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
ATP | - |
Mus musculus | |
| 1 | - |
AMP | cosubstrate ATP | Mus musculus | |
| 1 | - |
dAMP | cosubstrate ATP | Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | requirement, half as effective as Mg2+ or Mn2+ | Mus musculus | |
| Fe2+ | requirement, half as effective as Mg2+ or Mn2+ | Mus musculus | |
| Fe3+ | activation, only about 15% as effective as Mg2+ or Mn2+ | Mus musculus | |
| Mg2+ | requirement, as good as Mn2+ | Mus musculus | |
| Mn2+ | requirement, as good as Mg2+ | Mus musculus | |
| additional information | no activation by Ca2+, Zn2+ or KCl | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dAMP | Mus musculus | - |
ADP + dADP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fibroblast | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.6 | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + AMP | - |
Mus musculus | ADP + ADP | - |
? | |
| ATP + dAMP | - |
Mus musculus | ADP + dADP | - |
? | |
| ATP + dAMP | no substrates are GMP or dGMP | Mus musculus | ADP + dADP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
94%, 97%, 98% or 100% loss of activity within 2, 5, 20 or 30 min, respectively | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | broad, Tris-HCl preferred to 3,3-dimethylglutarate buffer | Mus musculus |
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