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Literature summary for 2.7.4.10 extracted from
- Nucleotide transphosphorylases from liver (1961), J. Biol. Chem., 236, 1800-1805.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | weak | Sus scrofa |  |
| HgCl2 | weak | Sus scrofa | |
| N-ethylmaleimide | weak | Sus scrofa | |
| p-hydroxymercuribenzoate | weak | Sus scrofa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | actual substrate: MgNTP | Sus scrofa | |
| Mn2+ | 75% as effective as Mg2+ | Sus scrofa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.5 | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C to 4°C, a few weeks | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dGTP + dAMP | - |
Sus scrofa | dGDP + dADP | - |
r | |
| GTP + AMP | no acceptor substrates are 2'-AMP, 3'-AMP | Sus scrofa | GDP + ADP | - |
r | |
| GTP + AMP | not: CMP, GMP, IMP, UMP | Sus scrofa | GDP + ADP | - |
r | |
| GTP + AMP | dAMP can replace AMP | Sus scrofa | GDP + ADP | - |
r | |
| ITP + AMP | dAMP can replace AMP | Sus scrofa | IDP + ADP | - |
r | |
| ITP + AMP | not: dCMP, dGMP, TDP, deoxyadenosine | Sus scrofa | IDP + ADP | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
5 min, 2 mg protein/ml, stable in 0.1 N HCl | Sus scrofa |
| 40 | - |
5 min, 2 mg protein/ml, in 0.1 N HCl, 70% loss of activity | Sus scrofa |
| 95 | - |
5 min, 95% inactivation at pH 7.4, 0.15 M imidazole buffer | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
IDP, ADP | Sus scrofa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | about half-maximal activity at pH 6 and about 80% of maximal activity at pH 9 | Sus scrofa |
| 6 | 9 | at pH 4.5 the activity is about 2% of that at pH 7.4 | Sus scrofa |
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Release 2023.1 (January 2023)
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