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Literature summary for 2.7.4.1 extracted from

  • Singh, M.; Tiwari, P.; Arora, G.; Agarwal, S.; Kidwai, S.; Singh, R.
    Establishing Virulence Associated Polyphosphate Kinase 2 as a drug target for Mycobacterium tuberculosis (2016), Sci. Rep., 6, 26900 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
F125A the proteins has significantly reduced activity as compared to wild type protein Mycobacterium tuberculosis
G72A the activity of MBP-G72APPK-2 and wild type proteins are similar Mycobacterium tuberculosis
W129A the proteins has significantly reduced activity as compared to wild type protein Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
NSC-30205 0.1 mM, inhibits more than 80%, cytotoxic to THP-1 macrophages with TC50 value 0.01 mM Mycobacterium tuberculosis
NSC-345647 0.1 mM, inhibits more than 80%, cytotoxic to THP-1 macrophages with TC50 value 0.005 mM Mycobacterium tuberculosis
NSC-35676 0.1 mM, inhibits more than 80%, non-cytotoxic to THP-1 cells even at 0.05 mM concentration Mycobacterium tuberculosis
NSC-9037 0.1 mM, inhibits more than 80%, non-cytotoxic to THP-1 cells even at 0.05 mM concentration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + (phosphate)n+1 Mycobacterium tuberculosis
-
 ATP + (phosphate)n
-
 ?
ADP + (phosphate)n+1 Mycobacterium tuberculosis H37Rv
-
 ATP + (phosphate)n
-
 ?
ADP + (phosphate)n+1 Mycobacterium tuberculosis ATCC 25618
-
 ATP + (phosphate)n
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis O05877
-
-
Mycobacterium tuberculosis H37Rv O05877
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (phosphate)3 the enzyme can utilize short chain polyphosphates, PolyP3 as the phosphate donor, however, its activity increases in the presence of long chain polyphosphates PolyP17 or PolyP45 Mycobacterium tuberculosis ATP + (phosphate)2
-
 ?
ADP + (phosphate)3 the enzyme can utilize short chain polyphosphates, PolyP3 as the phosphate donor, however, its activity increases in the presence of long chain polyphosphates PolyP17 or PolyP45 Mycobacterium tuberculosis H37Rv ATP + (phosphate)2
-
 ?
ADP + (phosphate)3 the enzyme can utilize short chain polyphosphates, PolyP3 as the phosphate donor, however, its activity increases in the presence of long chain polyphosphates PolyP17 or PolyP45 Mycobacterium tuberculosis ATCC 25618 ATP + (phosphate)2
-
 ?
ADP + (phosphate)n+1
-
 Mycobacterium tuberculosis ATP + (phosphate)n
-
 ?
ADP + (phosphate)n+1 the enzyme can utilize short chain polyphosphates, PolyP3 as the phosphate donor, however, its activity increases in the presence of long chain polyphosphates PolyP17 or PolyP45 Mycobacterium tuberculosis ATP + (phosphate)n
-
 ?
ADP + (phosphate)n+1
-
 Mycobacterium tuberculosis H37Rv ATP + (phosphate)n
-
 ?
ADP + (phosphate)n+1 the enzyme can utilize short chain polyphosphates, PolyP3 as the phosphate donor, however, its activity increases in the presence of long chain polyphosphates PolyP17 or PolyP45 Mycobacterium tuberculosis H37Rv ATP + (phosphate)n
-
 ?
ADP + (phosphate)n+1
-
 Mycobacterium tuberculosis ATCC 25618 ATP + (phosphate)n
-
 ?
ADP + (phosphate)n+1 the enzyme can utilize short chain polyphosphates, PolyP3 as the phosphate donor, however, its activity increases in the presence of long chain polyphosphates PolyP17 or PolyP45 Mycobacterium tuberculosis ATCC 25618 ATP + (phosphate)n
-
 ?

Synonyms

Synonyms Comment Organism
polyphosphate kinase 2
-
 Mycobacterium tuberculosis
PPK-2
-
 Mycobacterium tuberculosis
Rv3232c
-
 Mycobacterium tuberculosis

Expression

Organism Comment Expression
Mycobacterium tuberculosis a marginal decrease in ppk-2 transcript level is observed upon exposure of Mycobacterium tuberculosis to nutritional stress down
Mycobacterium tuberculosis ppk-2 transcripts are upregulated by 2.0-2.5 fold during mid-log, late-log and stationary phase stages of Mycobacterium tuberculosis growth in liquid cultures up

General Information

General Information Comment Organism
malfunction ppk-2 mutant strain infected guinea pigs have significantly reduced bacterial loads and tissue pathology in comparison to wild type infected guinea pigs at later stages of infection. The ppk-2 mutant strainis more tolerant to isoniazid and impaired for survival in THP-1 macrophages Mycobacterium tuberculosis
physiological function PPK-2 enzyme is required for Mycobacterium tuberculosis growth during acute and chronic stage of infection. PPK-2 enzyme contributes to the ability of Mycobacterium tuberculosis to cause disease in guinea pigs Mycobacterium tuberculosis