Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.4.1 extracted from

  • Motomura, K.; Hirota, R.; Okada, M.; Ikeda, T.; Ishida, T.; Kuroda, A.
    A new subfamily of polyphosphate kinase 2 (class III PPK2) catalyzes both nucleoside monophosphate phosphorylation and nucleoside diphosphate phosphorylation (2014), Appl. Environ. Microbiol., 80, 2602-2608 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.1

Protein Variants

Protein Variants Comment Organism
E126G about 18% of wild-type activity with substrate AMP, about 40% of wild-type activity with ADP Meiothermus ruber
E126N about 5% of wild-type activity with substrate AMP, bout 15% of wild-type activity with ADP Meiothermus ruber

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mn2+ is preferred over Mg2+ Meiothermus ruber
Mn2+ Mn2+ is preferred over Mg2+ Meiothermus ruber

Organism

Organism UniProt Comment Textmining
Meiothermus ruber M9XB82 cf. EC 2.7.4.1
-
Meiothermus ruber NBRC 106122 M9XB82 cf. EC 2.7.4.1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8
-
 substrate ADP, pH 7, 70°C Meiothermus ruber
99.1
-
 substrate AMP, pH 7, 70°C Meiothermus ruber

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (phosphate)n+1 enzyme prefers polyphosphate of 25 to 50 in chain length Meiothermus ruber ATP + (phosphate)n
-
 r
ADP + (phosphate)n+1 enzyme prefers polyphosphate of 25 to 50 in chain length Meiothermus ruber NBRC 106122 ATP + (phosphate)n
-
 r
AMP + (phosphate)n+2
-
 Meiothermus ruber ATP + (phosphate)n
-
 r
AMP + (phosphate)n+2
-
 Meiothermus ruber NBRC 106122 ATP + (phosphate)n
-
 r
ATP + (phosphate)n
-
 Meiothermus ruber ADP + (phosphate)n+1
-
 r
ATP + (phosphate)n
-
 Meiothermus ruber NBRC 106122 ADP + (phosphate)n+1
-
 r
additional information enzyme is able to use nucleoside diphosphates ADP, GDP, CDP, and UDP Meiothermus ruber ?
-
-
additional information enzyme is able to use nucleoside diphosphates ADP, GDP, CDP, and UDP Meiothermus ruber NBRC 106122 ?
-
-

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE, x * 31600, calculated from sequence Meiothermus ruber

Synonyms

Synonyms Comment Organism
K649_10090
-
 Meiothermus ruber
Mrub_2488
-
 Meiothermus ruber

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
 Meiothermus ruber

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 stable in the presence of both Mn2+ and polyphosphate but immediately loses its activity in the absence of either n2+ or polyphosphate Meiothermus ruber