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Literature summary for 2.7.4.1 extracted from

  • Nocek, B.; Khusnutdinova, A.; Ruszkowski, M.; Flick, R.; Burda, M.; Batyrova, K.; Brown, G.; Mucha, A.; Joachimiak, A.; Berlicki, L.; Yakunin, A.
    Structural insights into substrate selectivity and activity of bacterial polyphosphate kinases (2018), ACS Catal., 8, 10746-10760 .
No PubMed abstract available
Search for more literature for 2.7.4.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Sinorhizobium meliloti
-
 Cytophaga hutchinsonii
-
 Deinococcus radiodurans
-
 Paenarthrobacter aurescens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor-diffusion method at 18°C Sinorhizobium meliloti
sitting-drop vapor-diffusion method at 18°C Deinococcus radiodurans
sitting-drop vapor-diffusion method at 18°C Paenarthrobacter aurescens
sitting-drop vapor-diffusion method at 18°C, crystal structures of CHU0107 in complex with AMP, ADP, or guanosine 5'-tetraphosphate. The structures of CHU0107-inhibitor complexes reveales at least two binding pockets for aryl phosphonate inhibitors Cytophaga hutchinsonii

Inhibitors

Inhibitors Comment Organism Structure
guanosine 5'-tetraphosphate
-
 Cytophaga hutchinsonii
guanosine 5'-tetraphosphate
-
 Deinococcus radiodurans
guanosine 5'-tetraphosphate
-
 Paenarthrobacter aurescens
guanosine 5'-tetraphosphate
-
 Sinorhizobium meliloti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Paenarthrobacter aurescens
0.013
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Deinococcus radiodurans
0.11
-
 dADP pH 8.0, 30°C Paenarthrobacter aurescens
0.17
-
 ADP pH 8.0, 30°C Paenarthrobacter aurescens
0.2
-
 ADP pH 8.0, 30°C Deinococcus radiodurans
0.27
-
 AMP pH 8.0, 30°C Deinococcus radiodurans
0.29
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Cytophaga hutchinsonii
0.35
-
 ADP pH 8.0, 30°C Cytophaga hutchinsonii
0.53
-
 dADP pH 8.0, 30°C Cytophaga hutchinsonii
0.62
-
 AMP pH 8.0, 30°C Cytophaga hutchinsonii
0.71
-
 dAMP pH 8.0, 30°C Deinococcus radiodurans
0.83
-
 AMP pH 8.0, 30°C Paenarthrobacter aurescens
1.03
-
 dAMP pH 8.0, 30°C Paenarthrobacter aurescens
1.69
-
 dAMP pH 8.0, 30°C Cytophaga hutchinsonii
2.71
-
 ATP pH 8.0, 30°C Cytophaga hutchinsonii
2.74
-
 GDP pH 8.0, 30°C Cytophaga hutchinsonii
2.88
-
 GMP pH 8.0, 30°C Deinococcus radiodurans
3.15
-
 GMP pH 8.0, 30°C Cytophaga hutchinsonii
8.64
-
 ATP pH 8.0, 30°C Paenarthrobacter aurescens
17.7
-
 GMP pH 8.0, 30°C Paenarthrobacter aurescens
39.2
-
 GDP pH 8.0, 30°C Paenarthrobacter aurescens

Organism

Organism UniProt Comment Textmining
Cytophaga hutchinsonii A0A6N4SMB5
-
-
Cytophaga hutchinsonii ATCC 33406 A0A6N4SMB5
-
-
Deinococcus radiodurans Q9RY20
-
-
Deinococcus radiodurans ATCC 13939 Q9RY20
-
-
Paenarthrobacter aurescens A1R8G0
-
-
Paenarthrobacter aurescens TC1 A1R8G0
-
-
Sinorhizobium meliloti Q92SA6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sinorhizobium meliloti
-
 Cytophaga hutchinsonii
-
 Deinococcus radiodurans
-
 Paenarthrobacter aurescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (phosphate)n+1
-
 Cytophaga hutchinsonii ATP + (phosphate)n
-
 r
ADP + (phosphate)n+1
-
 Deinococcus radiodurans ATP + (phosphate)n
-
 ?
ADP + (phosphate)n+1
-
 Paenarthrobacter aurescens ATP + (phosphate)n
-
 r
ADP + (phosphate)n+1
-
 Cytophaga hutchinsonii ATCC 33406 ATP + (phosphate)n
-
 r
ADP + (phosphate)n+1
-
 Paenarthrobacter aurescens TC1 ATP + (phosphate)n
-
 r
ADP + (phosphate)n+1
-
 Deinococcus radiodurans ATCC 13939 ATP + (phosphate)n
-
 ?
AMP + (phosphate)n+1
-
 Cytophaga hutchinsonii ADP + (phosphate)n
-
 ?
AMP + (phosphate)n+1
-
 Deinococcus radiodurans ADP + (phosphate)n
-
 ?
AMP + (phosphate)n+1
-
 Paenarthrobacter aurescens ADP + (phosphate)n
-
 ?
AMP + (phosphate)n+1
-
 Cytophaga hutchinsonii ATCC 33406 ADP + (phosphate)n
-
 ?
AMP + (phosphate)n+1
-
 Paenarthrobacter aurescens TC1 ADP + (phosphate)n
-
 ?
AMP + (phosphate)n+1
-
 Deinococcus radiodurans ATCC 13939 ADP + (phosphate)n
-
 ?
ATP + (phosphate)n
-
 Cytophaga hutchinsonii ADP + (phosphate)n+1
-
 r
ATP + (phosphate)n
-
 Paenarthrobacter aurescens ADP + (phosphate)n+1
-
 r
ATP + (phosphate)n
-
 Cytophaga hutchinsonii ATCC 33406 ADP + (phosphate)n+1
-
 r
ATP + (phosphate)n
-
 Paenarthrobacter aurescens TC1 ADP + (phosphate)n+1
-
 r
dADP + (phosphate)n+1
-
 Cytophaga hutchinsonii dATP + (phosphate)n
-
 ?
dADP + (phosphate)n+1
-
 Paenarthrobacter aurescens dATP + (phosphate)n
-
 ?
dADP + (phosphate)n+1
-
 Cytophaga hutchinsonii ATCC 33406 dATP + (phosphate)n
-
 ?
dADP + (phosphate)n+1
-
 Paenarthrobacter aurescens TC1 dATP + (phosphate)n
-
 ?
dAMP + (phosphate)n+1
-
 Cytophaga hutchinsonii dADP + (phosphate)n
-
 ?
dAMP + (phosphate)n+1
-
 Deinococcus radiodurans dADP + (phosphate)n
-
 ?
dAMP + (phosphate)n+1
-
 Paenarthrobacter aurescens dADP + (phosphate)n
-
 ?
dAMP + (phosphate)n+1
-
 Deinococcus radiodurans ATCC 13939 dADP + (phosphate)n
-
 ?
GDP + (phosphate)n+1
-
 Cytophaga hutchinsonii GTP + (phosphate)n
-
 ?
GDP + (phosphate)n+1
-
 Paenarthrobacter aurescens GTP + (phosphate)n
-
 ?
GDP + (phosphate)n+1
-
 Cytophaga hutchinsonii ATCC 33406 GTP + (phosphate)n
-
 ?
GDP + (phosphate)n+1
-
 Paenarthrobacter aurescens TC1 GTP + (phosphate)n
-
 ?
GMP + (phosphate)n+1
-
 Cytophaga hutchinsonii GDP + (phosphate)n
-
 ?
GMP + (phosphate)n+1
-
 Deinococcus radiodurans GDP + (phosphate)n
-
 ?
GMP + (phosphate)n+1
-
 Paenarthrobacter aurescens GDP + (phosphate)n
-
 ?
GMP + (phosphate)n+1
-
 Deinococcus radiodurans ATCC 13939 GDP + (phosphate)n
-
 ?

Synonyms

Synonyms Comment Organism
AAur2811
-
 Paenarthrobacter aurescens
CHU0107
-
 Cytophaga hutchinsonii
DR0132
-
 Deinococcus radiodurans
PPK2
-
 Sinorhizobium meliloti
PPK2
-
 Cytophaga hutchinsonii
PPK2
-
 Deinococcus radiodurans
PPK2
-
 Paenarthrobacter aurescens
SMc02148
-
 Sinorhizobium meliloti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 dAMP pH 8.0, 30°C Deinococcus radiodurans
0.29
-
 ATP pH 8.0, 30°C Cytophaga hutchinsonii
0.4
-
 ADP pH 8.0, 30°C Deinococcus radiodurans
0.55
-
 dADP pH 8.0, 30°C Paenarthrobacter aurescens
0.58
-
 dADP pH 8.0, 30°C Cytophaga hutchinsonii
0.6
-
 dAMP pH 8.0, 30°C Paenarthrobacter aurescens
0.8
-
 GMP pH 8.0, 30°C Deinococcus radiodurans
0.97
-
 GMP pH 8.0, 30°C Paenarthrobacter aurescens
2.12
-
 GDP pH 8.0, 30°C Paenarthrobacter aurescens
2.77
-
 dAMP pH 8.0, 30°C Cytophaga hutchinsonii
3.32
-
 ATP pH 8.0, 30°C Paenarthrobacter aurescens
3.86
-
 GDP pH 8.0, 30°C Cytophaga hutchinsonii
4.12
-
 ADP pH 8.0, 30°C Paenarthrobacter aurescens
5.53
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Paenarthrobacter aurescens
6.91
-
 GMP pH 8.0, 30°C Cytophaga hutchinsonii
9.39
-
 ADP pH 8.0, 30°C Cytophaga hutchinsonii
16.83
-
 AMP pH 8.0, 30°C Cytophaga hutchinsonii
21.24
-
 AMP pH 8.0, 30°C Paenarthrobacter aurescens
29.63
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Cytophaga hutchinsonii
94.47
-
 AMP pH 8.0, 30°C Deinococcus radiodurans
132
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Deinococcus radiodurans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
 ATP pH 8.0, 30°C Cytophaga hutchinsonii
0.1
-
 GDP pH 8.0, 30°C Paenarthrobacter aurescens
0.1
-
 GMP pH 8.0, 30°C Paenarthrobacter aurescens
0.1
-
 dAMP pH 8.0, 30°C Deinococcus radiodurans
0.3
-
 GMP pH 8.0, 30°C Deinococcus radiodurans
0.4
-
 ATP pH 8.0, 30°C Paenarthrobacter aurescens
0.6
-
 dAMP pH 8.0, 30°C Paenarthrobacter aurescens
1.1
-
 dADP pH 8.0, 30°C Cytophaga hutchinsonii
1.4
-
 GDP pH 8.0, 30°C Cytophaga hutchinsonii
1.6
-
 dAMP pH 8.0, 30°C Cytophaga hutchinsonii
2
-
 ADP pH 8.0, 30°C Deinococcus radiodurans
2.2
-
 GMP pH 8.0, 30°C Cytophaga hutchinsonii
5
-
 dADP pH 8.0, 30°C Paenarthrobacter aurescens
24
-
 ADP pH 8.0, 30°C Paenarthrobacter aurescens
26
-
 AMP pH 8.0, 30°C Paenarthrobacter aurescens
27
-
 ADP pH 8.0, 30°C Cytophaga hutchinsonii
27
-
 AMP pH 8.0, 30°C Cytophaga hutchinsonii
100
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Cytophaga hutchinsonii
350
-
 AMP pH 8.0, 30°C Deinococcus radiodurans
500
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Paenarthrobacter aurescens
10200
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Deinococcus radiodurans