Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycolicibacterium smegmatis |
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
F67A | 84% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
G69R | 67.1% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
G74R | 7.6% activity compared to the wild type enzyme, the mutant enzyme cannot interact with nucleoside diphosphate kinase | Mycobacterium tuberculosis |
H115A | 49% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
H115A/H247A | 6% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
H247A | 38% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
K75A | 89% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mycolicibacterium smegmatis | 16020 | - |
membrane | - |
Mycobacterium tuberculosis | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycolicibacterium smegmatis | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | ? | - |
? | |
additional information | Mycobacterium tuberculosis | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | ? | - |
? | |
additional information | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Mycolicibacterium smegmatis | - |
- |
- |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose column chromatography | Mycolicibacterium smegmatis |
Ni2+-NTA agarose column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + (phosphate)n+1 | PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro | Mycolicibacterium smegmatis | GTP + (phosphate)n | - |
? | |
GDP + (phosphate)n+1 | PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro | Mycobacterium tuberculosis | GTP + (phosphate)n | - |
? | |
GDP + (phosphate)n+1 | PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | GTP + (phosphate)n | - |
? | |
GDP + (phosphate)n+1 | PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro | Mycobacterium tuberculosis H37Rv | GTP + (phosphate)n | - |
? | |
additional information | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | Mycolicibacterium smegmatis | ? | - |
? | |
additional information | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | Mycobacterium tuberculosis | ? | - |
? | |
additional information | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? | |
additional information | PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphosphate kinase 2 | - |
Mycolicibacterium smegmatis |
polyphosphate kinase 2 | - |
Mycobacterium tuberculosis |
PPK2 | - |
Mycolicibacterium smegmatis |
PPK2 | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
GDP | - |
Mycolicibacterium smegmatis | |
GDP | - |
Mycobacterium tuberculosis |