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Literature summary for 2.7.4.1 extracted from

  • Sureka, K.; Sanyal, S.; Basu, J.; Kundu, M.
    Polyphosphate kinase 2: a modulator of nucleoside diphosphate kinase activity in mycobacteria (2009), Mol. Microbiol., 74, 1187-1197.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycolicibacterium smegmatis
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
F67A 84% activity compared to the wild type enzyme Mycobacterium tuberculosis
G69R 67.1% activity compared to the wild type enzyme Mycobacterium tuberculosis
G74R 7.6% activity compared to the wild type enzyme, the mutant enzyme cannot interact with nucleoside diphosphate kinase Mycobacterium tuberculosis
H115A 49% activity compared to the wild type enzyme Mycobacterium tuberculosis
H115A/H247A 6% activity compared to the wild type enzyme Mycobacterium tuberculosis
H247A 38% activity compared to the wild type enzyme Mycobacterium tuberculosis
K75A 89% activity compared to the wild type enzyme Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Mycolicibacterium smegmatis 16020
-
membrane
-
Mycobacterium tuberculosis 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycolicibacterium smegmatis PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase ?
-
?
additional information Mycobacterium tuberculosis PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase ?
-
?
additional information Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase ?
-
?
additional information Mycobacterium tuberculosis H37Rv PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase ?
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-
Mycolicibacterium smegmatis
-
-
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA agarose column chromatography Mycolicibacterium smegmatis
Ni2+-NTA agarose column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + (phosphate)n+1 PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro Mycolicibacterium smegmatis GTP + (phosphate)n
-
?
GDP + (phosphate)n+1 PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro Mycobacterium tuberculosis GTP + (phosphate)n
-
?
GDP + (phosphate)n+1 PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 GTP + (phosphate)n
-
?
GDP + (phosphate)n+1 PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro Mycobacterium tuberculosis H37Rv GTP + (phosphate)n
-
?
additional information PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase Mycolicibacterium smegmatis ?
-
?
additional information PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase Mycobacterium tuberculosis ?
-
?
additional information PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
?
additional information PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase Mycobacterium tuberculosis H37Rv ?
-
?

Synonyms

Synonyms Comment Organism
polyphosphate kinase 2
-
Mycolicibacterium smegmatis
polyphosphate kinase 2
-
Mycobacterium tuberculosis
PPK2
-
Mycolicibacterium smegmatis
PPK2
-
Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
GDP
-
Mycolicibacterium smegmatis
GDP
-
Mycobacterium tuberculosis