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Literature summary for 2.7.4.1 extracted from
- Crystallization and characterization of polyphosphate kinase from Escherichia coli (2003), Biochem. Biophys. Res. Commun., 305, 997-1001.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method at room temperatur, long rod crystals appear after 3 days in a mixture of 0.005 ml protein and 0.005 ml reservoir solution consisting of 12-14% hexandiol, 10 mM dithiothreitol, 100 mM Tris-HCl, pH 7.5, crystals of polyphosphate kinase complexed with AMP-PNP diffract to 2.5 A resolution | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 275000 | - |
dynamic light scattering | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged polyphosphate kinase, Ni-NTA affinity column | Escherichia coli |
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Release 2023.1 (January 2023)
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