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Literature summary for 2.7.4.1 extracted from
- A nonradioactive method for the assay of polyphosphate kinase activity and its application in the study of polyphosphate metabolism in Burkholderia cepacia (2002), Anal. Biochem., 308, 294-299.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 0.025 mM, 0.05 mM, 0.1 mM and 0.25 mM, 56%, 68%, 77% and 100% inhibition, respectively of polyphosphate synthesis in crude extracts | Burkholderia cepacia |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.83 | - |
ATP | pH 7.0, 20°C, polyphosphate kinase activity in cell extracts, detrmined with Robinson and Wood method | Burkholderia cepacia | |
| 1.18 | - |
ATP | pH 7.0, 20°C, polyphosphate kinase activity in cell extracts, kinetic analysis | Burkholderia cepacia | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal activation at 10 mM | Burkholderia cepacia | |
| Mn2+ | can replace Mg2+ to some extent | Burkholderia cepacia | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.13 | - |
polyphosphate kinase activity in crude extracts measured with a modified toluidine blue assay | Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | no activity with CTP, GTP and TTP | Burkholderia cepacia | ADP + (phosphate)n+1 | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Burkholderia cepacia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Burkholderia cepacia |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | approx. 10% of maximal activity at pH 5.5 and pH 8.0 respectively | Burkholderia cepacia |
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Release 2023.1 (January 2023)
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