Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | only active as glycogen-enzyme complex | Sulfolobus acidocaldarius |
General Stability | Organism |
---|---|
enzymatic hydrolysis of glycogen inactivates | Sulfolobus acidocaldarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 2 mM, 50% inhibition of glycogen-bound polyphosphate kinase; product inhibition, about 50% inhibition at 2 mM | Sulfolobus acidocaldarius | |
ammonium hydrochloride | 50% inhibition at 200 mM | Sulfolobus acidocaldarius | |
ATP | 20% substrate inhibition at 2.4 mM | Sulfolobus acidocaldarius | |
F- | 2 mM, 25% inhibition of glycogen-bound polyphosphate kinase | Sulfolobus acidocaldarius | |
hydrolyzed glycogen | complete inhibition | Sulfolobus acidocaldarius | |
additional information | enzymatic hydrolysis of glycogen | Sulfolobus acidocaldarius | |
NH4Cl | 200 mM, approx. 50% inhibition of glycogen-bound polyphosphate kinase | Sulfolobus acidocaldarius | |
phosphate | 2 mM, 50% inhibition of glycogen-bound polyphosphate kinase; strong inhibition | Sulfolobus acidocaldarius | |
Polyphosphate | substrate inhibition at 2 mM | Sulfolobus acidocaldarius | |
Sodium fluoride | slight inhibition | Sulfolobus acidocaldarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | exponential kinetics | Sulfolobus acidocaldarius | |
0.075 | - |
ATP | pH 7.0, 75°C | Sulfolobus acidocaldarius | |
0.075 | - |
ATP | pH 7.0, 75°C, glycogen-complexed enzyme | Sulfolobus acidocaldarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | glycogen-bound | Sulfolobus acidocaldarius | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activation | Sulfolobus acidocaldarius | |
KCl | activates | Sulfolobus acidocaldarius | |
Mg2+ | stimulates | Sulfolobus acidocaldarius | |
Mg2+ | activation | Sulfolobus acidocaldarius | |
Mg2+ | 50% as effective as Mn2+ | Sulfolobus acidocaldarius | |
Mn2+ | maximal activity at 2 mM | Sulfolobus acidocaldarius | |
Mn2+ | stimulates, preferred metal ion | Sulfolobus acidocaldarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, glycogen-complexed enzyme, SDS-PAGE | Sulfolobus acidocaldarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | Sulfolobus acidocaldarius | - |
ADP + (phosphate)n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | - |
- |
- |
Sulfolobus acidocaldarius | - |
strain DSM 639 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | glycogen-bound polyphosphate kinase | Sulfolobus acidocaldarius |
Purification (Comment) | Organism |
---|---|
native enzyme as glycogen complex partially from crude extract by isopycnic centrifugation in CsCl, followed by dialysis | Sulfolobus acidocaldarius |
partial purification | Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | - |
Sulfolobus acidocaldarius | ADP + (phosphate)n+1 | - |
? | |
ATP + (phosphate)n | glycogen-bound polyphosphate kinase | Sulfolobus acidocaldarius | ADP + (phosphate)n+1 | - |
? | |
ATP + (phosphate)n | the enzyme does not require the presence of histones for its activity | Sulfolobus acidocaldarius | ADP + (phosphate)n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, glycogen-complexed enzyme, SDS-PAGE | Sulfolobus acidocaldarius |
Synonyms | Comment | Organism |
---|---|---|
glycogen-bound polyphosphate kinase | - |
Sulfolobus acidocaldarius |
poly-P kinase | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Sulfolobus acidocaldarius |
75 | - |
assay at | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Sulfolobus acidocaldarius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sulfolobus acidocaldarius |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sulfolobus acidocaldarius | several spots, isoelectric focusing | 6.7 | 5.6 |