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Literature summary for 2.7.4.1 extracted from
- Glycogen-bound polyphosphate kinase from the archaebacterium Sulfolobus acidocaldarius (1989), J. Bacteriol., 171, 5162-5164.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | only active as glycogen-enzyme complex | Sulfolobus acidocaldarius |
General Stability
| General Stability | Organism |
|---|---|
| enzymatic hydrolysis of glycogen inactivates | Sulfolobus acidocaldarius |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 2 mM, 50% inhibition of glycogen-bound polyphosphate kinase; product inhibition, about 50% inhibition at 2 mM | Sulfolobus acidocaldarius |  |
| ammonium hydrochloride | 50% inhibition at 200 mM | Sulfolobus acidocaldarius | |
| ATP | 20% substrate inhibition at 2.4 mM | Sulfolobus acidocaldarius | |
| F- | 2 mM, 25% inhibition of glycogen-bound polyphosphate kinase | Sulfolobus acidocaldarius | |
| hydrolyzed glycogen | complete inhibition | Sulfolobus acidocaldarius | |
| additional information | enzymatic hydrolysis of glycogen | Sulfolobus acidocaldarius | |
| NH4Cl | 200 mM, approx. 50% inhibition of glycogen-bound polyphosphate kinase | Sulfolobus acidocaldarius | |
| phosphate | 2 mM, 50% inhibition of glycogen-bound polyphosphate kinase; strong inhibition | Sulfolobus acidocaldarius | |
| Polyphosphate | substrate inhibition at 2 mM | Sulfolobus acidocaldarius | |
| Sodium fluoride | slight inhibition | Sulfolobus acidocaldarius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | exponential kinetics | Sulfolobus acidocaldarius | |
| 0.075 | - |
ATP | pH 7.0, 75°C | Sulfolobus acidocaldarius | |
| 0.075 | - |
ATP | pH 7.0, 75°C, glycogen-complexed enzyme | Sulfolobus acidocaldarius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | glycogen-bound | Sulfolobus acidocaldarius | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activation | Sulfolobus acidocaldarius | |
| KCl | activates | Sulfolobus acidocaldarius | |
| Mg2+ | stimulates | Sulfolobus acidocaldarius | |
| Mg2+ | activation | Sulfolobus acidocaldarius | |
| Mg2+ | 50% as effective as Mn2+ | Sulfolobus acidocaldarius | |
| Mn2+ | maximal activity at 2 mM | Sulfolobus acidocaldarius | |
| Mn2+ | stimulates, preferred metal ion | Sulfolobus acidocaldarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
x * 57000, glycogen-complexed enzyme, SDS-PAGE | Sulfolobus acidocaldarius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | Sulfolobus acidocaldarius | - |
ADP + (phosphate)n+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | - |
- |
- |
| Sulfolobus acidocaldarius | - |
strain DSM 639 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | glycogen-bound polyphosphate kinase | Sulfolobus acidocaldarius |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme as glycogen complex partially from crude extract by isopycnic centrifugation in CsCl, followed by dialysis | Sulfolobus acidocaldarius |
| partial purification | Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | - |
Sulfolobus acidocaldarius | ADP + (phosphate)n+1 | - |
? | |
| ATP + (phosphate)n | glycogen-bound polyphosphate kinase | Sulfolobus acidocaldarius | ADP + (phosphate)n+1 | - |
? | |
| ATP + (phosphate)n | the enzyme does not require the presence of histones for its activity | Sulfolobus acidocaldarius | ADP + (phosphate)n+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000, glycogen-complexed enzyme, SDS-PAGE | Sulfolobus acidocaldarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycogen-bound polyphosphate kinase | - |
Sulfolobus acidocaldarius |
| poly-P kinase | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Sulfolobus acidocaldarius |
| 75 | - |
assay at | Sulfolobus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Sulfolobus acidocaldarius |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Sulfolobus acidocaldarius | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sulfolobus acidocaldarius | several spots, isoelectric focusing | 6.7 | 5.6 |
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