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Literature summary for 2.7.4.1 extracted from

  • Robinson, N.A.; Clark, J.E.; Wood, H.G.
    Polyphosphate kinase from Propionibacterium shermanii. Demonstration that polyphosphates are primers and determination of the size of the synthesized polyphosphate (1987), J. Biol. Chem., 262, 5216-5222.
    View publication on PubMed
Search for more literature for 2.7.4.1

Activating Compound

Activating Compound Comment Organism Structure
phosphate 10fold higher rate of polyphosphate synthesis Propionibacterium freudenreichii subsp. shermanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
 native PAGE Propionibacterium freudenreichii subsp. shermanii
85000
-
 gel filtration Propionibacterium freudenreichii subsp. shermanii

Organism

Organism UniProt Comment Textmining
Propionibacterium freudenreichii subsp. shermanii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DE52, hydroxylapatite Propionibacterium freudenreichii subsp. shermanii

Reaction

Reaction Comment Organism Reaction ID
ATP + (phosphate)n = ADP + (phosphate)n+1 short chain polyphosphates are incorporated at the ends of the long chain product Propionibacterium freudenreichii subsp. shermanii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.73
-
-
 Propionibacterium freudenreichii subsp. shermanii

Storage Stability

Storage Stability Organism
4°C, in 20 mM Tris-HCl buffer, pH 7.6, 200 mM NaCl, 10% glycerol, 1 mM EDTA, 0.7 mM 2-mercaptoethanol, at least 1 month Propionibacterium freudenreichii subsp. shermanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (phosphate)n strictly processive mechanism of polyphosphate elongation, phosphate or short-chains of polyphosphates serve as primers, majority of the synthesized polyphosphates is 750 residues long Propionibacterium freudenreichii subsp. shermanii ADP + (phosphate)n+1 polyphosphate as primer: product with 750 residues, phosphate as primer: product with 300-2000 residues, i.e. major form with chain length of 2000 ?

Subunits

Subunits Comment Organism
monomer 1 * 85000, SDS-PAGE Propionibacterium freudenreichii subsp. shermanii