Literature summary for 2.7.4.1 extracted from

Haeusler, P.A.; Dieter, L.; Rittle, K.J.; Shepler, L.S.; Paszkowski, A.L.; Moe, O.A.
Catalytic properties of Escherichia coli polyphosphate kinase: an enzyme for ATP regeneration (1992), Biotechnol. Appl. Biochem., 15, 125-133.

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Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	1 mM, 10% inhibition	Escherichia coli
Co2+	1 mM, 40% inhibition	Escherichia coli
Cu2+	0.1 mM, 92% inhibition, 1 mM, complete inhibition	Escherichia coli
Mn2+	weak inhibition at 10 mM Mg2+	Escherichia coli
Zn2+	0.1 mM, 67% inhibition, 1 mM, complete inhibition	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.072	-	ADP	pH 5.5, 25°C	Escherichia coli
0.18	-	ADP	pH 6.5-8.5, 25°C	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	1.5-2.5fold lower activation than with Mg2+, inhibitory with Mg2+ as activator	Escherichia coli
Mg2+	activation	Escherichia coli
Mg2+	maximal activity at 10 mM	Escherichia coli
Mn2+	activation, can replace Mg2+	Escherichia coli
Mn2+	1.5-2.5fold lower activation than with Mg2+	Escherichia coli
additional information	not activated by Ca2+	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial purification	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + (phosphate)n+1	-	Escherichia coli	ATP + (phosphate)n	-	r
ATP + (phosphate)n	best substrates are polyphosphates with more than 132 residues	Escherichia coli	ADP + (phosphate)n+1	-	r
ATP + (phosphate)n	no activity with phosphates of 5 residues or below	Escherichia coli	ADP + (phosphate)n+1	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	-	Escherichia coli

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	8.5	approx. 19% of maximal activity at pH 5.5 and pH 8.5	Escherichia coli

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Release 2023.1 (January 2023)