Cloned (Comment) | Organism |
---|---|
gene PHYSODRAFT_566100, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of N-terminal His6-PsPK protein in Escherichia coli strain Rosetta 2 (DE3) | Phytophthora sojae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis, overview. Analysis of quaternary structure and cooperativity in ligand-binding by ITC: absence of inter-subunit cooperativity in forming the PsTKTSAC | Phytophthora sojae | |
0.52 | - |
Taurocyamine | recombinant enzyme, pH 9.0, 30°C | Phytophthora sojae | |
1.8 | - |
ATP | recombinant enzyme, pH 9.0, 30°C, with taurocyamine | Phytophthora sojae | |
4.6 | - |
ATP | recombinant enzyme, pH 9.0, 30°C, with glycocyamine | Phytophthora sojae | |
6.1 | - |
glycocyamine | recombinant enzyme, pH 9.0, 30°C | Phytophthora sojae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Phytophthora sojae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycocyamine | Phytophthora sojae | low activity | ADP + N-phosphoglycocyamine | - |
? | |
ATP + glycocyamine | Phytophthora sojae P6497 | low activity | ADP + N-phosphoglycocyamine | - |
? | |
ATP + taurocyamine | Phytophthora sojae | - |
ADP + N-phosphotaurocyamine | - |
? | |
ATP + taurocyamine | Phytophthora sojae P6497 | - |
ADP + N-phosphotaurocyamine | - |
? | |
additional information | Phytophthora sojae | the phosphagen kinases in the invertebrates are observed to utilize other guanidino substrates. These include arginine, glycocyamine, taurocyamine, lombricine, hypotaurocyamine, and opheline. Analysis of phosphagen formation by 31P NMR, overview | ? | - |
? | |
additional information | Phytophthora sojae P6497 | the phosphagen kinases in the invertebrates are observed to utilize other guanidino substrates. These include arginine, glycocyamine, taurocyamine, lombricine, hypotaurocyamine, and opheline. Analysis of phosphagen formation by 31P NMR, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phytophthora sojae | G5ADV1 | gene PHYSODRAFT_566100 | - |
Phytophthora sojae P6497 | G5ADV1 | gene PHYSODRAFT_566100 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminal His6-PsPK protein from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration | Phytophthora sojae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycocyamine | low activity | Phytophthora sojae | ADP + N-phosphoglycocyamine | - |
? | |
ATP + glycocyamine | low activity | Phytophthora sojae P6497 | ADP + N-phosphoglycocyamine | - |
? | |
ATP + taurocyamine | - |
Phytophthora sojae | ADP + N-phosphotaurocyamine | - |
? | |
ATP + taurocyamine | - |
Phytophthora sojae P6497 | ADP + N-phosphotaurocyamine | - |
? | |
additional information | the phosphagen kinases in the invertebrates are observed to utilize other guanidino substrates. These include arginine, glycocyamine, taurocyamine, lombricine, hypotaurocyamine, and opheline. Analysis of phosphagen formation by 31P NMR, overview | Phytophthora sojae | ? | - |
? | |
additional information | no kinase activity with the more ubiquitous guanidinium substrates, creatine or arginine | Phytophthora sojae | ? | - |
? | |
additional information | the phosphagen kinases in the invertebrates are observed to utilize other guanidino substrates. These include arginine, glycocyamine, taurocyamine, lombricine, hypotaurocyamine, and opheline. Analysis of phosphagen formation by 31P NMR, overview | Phytophthora sojae P6497 | ? | - |
? | |
additional information | no kinase activity with the more ubiquitous guanidinium substrates, creatine or arginine | Phytophthora sojae P6497 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 + 51000, about, sequence calculation, the enzyme is dimeric but lacks cooperativity between the subunits in forming a transition state analogue complex | Phytophthora sojae |
Synonyms | Comment | Organism |
---|---|---|
hypotaurocyamine kinase | - |
Phytophthora sojae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Phytophthora sojae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
glycocyamine | recombinant enzyme, pH 9.0, 30°C | Phytophthora sojae | |
94.1 | - |
Taurocyamine | recombinant enzyme, pH 9.0, 30°C | Phytophthora sojae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Phytophthora sojae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Phytophthora sojae |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the phosphagen kinases family of enzymes. Phylogenetic analysis of oomycete enzymes, overview | Phytophthora sojae |
physiological function | within animal species, these enzymes play a critical role in energy homeostasis by catalyzing the reversible transfer of a high-energy phosphoryl group from Mg-ATP to an acceptor molecule containing a guanidinium group | Phytophthora sojae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
glycocyamine | recombinant enzyme, pH 9.0, 30°C | Phytophthora sojae | |
180 | - |
Taurocyamine | recombinant enzyme, pH 9.0, 30°C | Phytophthora sojae |