BRENDA - Enzyme Database
show all sequences of 2.7.3.4

Phosphagen kinase of the giant tubeworm Riftia pachyptila. Cloning and expression of cytoplasmic and mitochondrial isoforms of taurocyamine kinase

Uda, K.; Tanaka, K.; Bailly, X.; Zal, F.; Suzuki, T.; Int. J. Biol. Macromol. 37, 54-60 (2005)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli TB1; expression in Escherichia coli TB1
Riftia pachyptila
General Stability
General Stability
Organism
structurally very unstable, gradual loss of enzyme activity even when it is stored on ice for several hours
Riftia pachyptila
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.881
-
Taurocyamine
-
Arenicola brasiliensis
2.12
-
Taurocyamine
-
Riftia pachyptila
4.01
-
Taurocyamine
-
Arenicola brasiliensis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Arenicola brasiliensis
5737
-
cytoplasm
-
Riftia pachyptila
5737
-
mitochondrion
-
Arenicola brasiliensis
5739
-
mitochondrion
-
Riftia pachyptila
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40830
-
calculated from amino acid sequence
Riftia pachyptila
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + taurocyamine
Riftia pachyptila
key role in the interconnection between energy production and utilization in animals
ADP + N-phosphotaurocyamine
-
-
?
ATP + taurocyamine
Arenicola brasiliensis
key role in the interconnection between energy production and utilization in animals
ADP + N-phosphotaurocyamine
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Arenicola brasiliensis
-
-
-
Riftia pachyptila
Q4AEC7
-
-
Riftia pachyptila
Q4AEC8
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant protein is purified by affinity chromatography using amylose resin; recombinant protein is purified by affinity chromatography using amylose resin
Riftia pachyptila
Source Tissue
Source Tissue
Commentary
Organism
Textmining
plume
a small part of the plume; a small part of the plume
Riftia pachyptila
-
vestimentum
;
Riftia pachyptila
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.298
-
arginine
Riftia pachyptila
0.855
-
lombricine
Riftia pachyptila
3.25
-
lombricine
Riftia pachyptila
3.65
-
glycocyamine
Riftia pachyptila
4.04
-
taurocyamine
Riftia pachyptila
10.4
-
taurocyamine
Riftia pachyptila
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + arginine
2.9% of taurocyamine activity
661899
Riftia pachyptila
ADP + ?
-
-
-
?
ATP + glycocyamine
no activity
661899
Riftia pachyptila
ADP + N-phosphoglycocyamine
-
-
-
?
ATP + glycocyamine
35% of taurocyamine activity
661899
Riftia pachyptila
ADP + N-phosphoglycocyamine
-
-
-
?
ATP + lombricine
21% of taurocyamine activity
661899
Riftia pachyptila
ADP + N-phospholombricine
-
-
-
?
ATP + lombricine
31% of taurocyamine activity
661899
Riftia pachyptila
ADP + N-phospholombricine
-
-
-
?
ATP + taurocyamine
-
661899
Riftia pachyptila
ADP + N-phosphotaurocyamine
-
-
-
?
ATP + taurocyamine
-
661899
Arenicola brasiliensis
ADP + N-phosphotaurocyamine
-
-
-
?
ATP + taurocyamine
key role in the interconnection between energy production and utilization in animals
661899
Riftia pachyptila
ADP + N-phosphotaurocyamine
-
-
-
?
ATP + taurocyamine
key role in the interconnection between energy production and utilization in animals
661899
Arenicola brasiliensis
ADP + N-phosphotaurocyamine
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
MiTK
-
Arenicola brasiliensis
phosphagen kinase
-
Riftia pachyptila
taurocyamine kinase
-
Arenicola brasiliensis
taurocyamine kinase
-
Riftia pachyptila
TK
-
Arenicola brasiliensis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9.43
-
Taurocyamine
-
Arenicola brasiliensis
12.5
-
Taurocyamine
-
Riftia pachyptila
14.3
-
Taurocyamine
-
Arenicola brasiliensis
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Riftia pachyptila
calculated
-
7.98
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli TB1
Riftia pachyptila
General Stability (protein specific)
General Stability
Organism
structurally very unstable, gradual loss of enzyme activity even when it is stored on ice for several hours
Riftia pachyptila
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.881
-
Taurocyamine
-
Arenicola brasiliensis
2.12
-
Taurocyamine
-
Riftia pachyptila
4.01
-
Taurocyamine
-
Arenicola brasiliensis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Arenicola brasiliensis
5737
-
cytoplasm
-
Riftia pachyptila
5737
-
mitochondrion
-
Arenicola brasiliensis
5739
-
mitochondrion
-
Riftia pachyptila
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40830
-
calculated from amino acid sequence
Riftia pachyptila
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + taurocyamine
Riftia pachyptila
key role in the interconnection between energy production and utilization in animals
ADP + N-phosphotaurocyamine
-
-
?
ATP + taurocyamine
Arenicola brasiliensis
key role in the interconnection between energy production and utilization in animals
ADP + N-phosphotaurocyamine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein is purified by affinity chromatography using amylose resin
Riftia pachyptila
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
plume
a small part of the plume
Riftia pachyptila
-
vestimentum
-
Riftia pachyptila
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.298
-
arginine
Riftia pachyptila
0.855
-
lombricine
Riftia pachyptila
3.25
-
lombricine
Riftia pachyptila
3.65
-
glycocyamine
Riftia pachyptila
4.04
-
taurocyamine
Riftia pachyptila
10.4
-
taurocyamine
Riftia pachyptila
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + arginine
2.9% of taurocyamine activity
661899
Riftia pachyptila
ADP + ?
-
-
-
?
ATP + glycocyamine
no activity
661899
Riftia pachyptila
ADP + N-phosphoglycocyamine
-
-
-
?
ATP + glycocyamine
35% of taurocyamine activity
661899
Riftia pachyptila
ADP + N-phosphoglycocyamine
-
-
-
?
ATP + lombricine
21% of taurocyamine activity
661899
Riftia pachyptila
ADP + N-phospholombricine
-
-
-
?
ATP + lombricine
31% of taurocyamine activity
661899
Riftia pachyptila
ADP + N-phospholombricine
-
-
-
?
ATP + taurocyamine
-
661899
Riftia pachyptila
ADP + N-phosphotaurocyamine
-
-
-
?
ATP + taurocyamine
-
661899
Arenicola brasiliensis
ADP + N-phosphotaurocyamine
-
-
-
?
ATP + taurocyamine
key role in the interconnection between energy production and utilization in animals
661899
Riftia pachyptila
ADP + N-phosphotaurocyamine
-
-
-
?
ATP + taurocyamine
key role in the interconnection between energy production and utilization in animals
661899
Arenicola brasiliensis
ADP + N-phosphotaurocyamine
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9.43
-
Taurocyamine
-
Arenicola brasiliensis
12.5
-
Taurocyamine
-
Riftia pachyptila
14.3
-
Taurocyamine
-
Arenicola brasiliensis
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Riftia pachyptila
calculated
-
7.98
Other publictions for EC 2.7.3.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738646
Merceron
The substrate-free and -bound ...
Schistosoma mansoni
J. Biol. Chem.
290
12951-12963
2015
-
-
1
1
3
-
-
-
-
1
-
1
-
3
-
-
1
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
3
-
-
-
-
-
-
1
-
1
-
-
-
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722083
Uda
A novel taurocyamine kinase fo ...
Phytophthora infestans
Comp. Biochem. Physiol. B
165
42-48
2013
-
-
-
-
-
-
-
2
-
-
2
-
-
4
-
-
-
-
-
1
-
-
3
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738077
Palmer
Characterization of a putative ...
Phytophthora sojae, Phytophthora sojae P6497
Comp. Biochem. Physiol. B
166
173-181
2013
-
-
1
-
-
-
-
5
-
1
-
6
-
4
-
-
1
-
-
-
-
-
8
1
1
1
-
-
2
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
-
1
-
6
-
-
-
1
-
-
-
-
8
1
1
-
-
2
1
-
-
-
-
2
2
-
2
2
738180
Jarilla
The role of Y84 on domain 1 an ...
Paragonimus westermani
Exp. Parasitol.
135
695-700
2013
-
-
1
-
14
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
14
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
739402
Xiao
Molecular cloning and characte ...
Clonorchis sinensis
PLoS Negl. Trop. Dis.
7
e2548
2013
-
1
1
-
8
-
-
7
-
1
5
1
-
4
-
-
1
-
-
8
-
-
3
1
1
1
-
-
6
1
-
-
1
-
3
-
-
1
1
1
-
8
-
-
-
-
7
-
1
5
1
-
-
-
1
-
8
-
-
3
1
1
-
-
6
1
-
-
3
-
1
1
-
-
-
721715
Tanaka
Identification of amino acid r ...
Arenicola brasiliensis
Biochim. Biophys. Acta
1814
1219-1225
2011
-
-
1
-
18
-
-
19
3
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
18
-
-
-
-
19
3
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703716
Jarilla
Molecular characterization and ...
Paragonimus westermani
FEBS Lett.
583
2218-2224
2009
-
-
1
-
-
-
-
3
-
-
1
-
-
3
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
3
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
704055
Uda
The role of Arg-96 in Danio re ...
Danio rerio
Int. J. Biol. Macromol.
44
413-418
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690293
Awama
Crystallization and x-ray anal ...
Schistosoma mansoni
Acta Crystallogr. Sect. F
F64
854-857
2008
-
1
-
1
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691916
Suzuki
Evolution of the diverse array ...
Arenicola brasiliensis
Comp. Biochem. Physiol. B
152
60-66
2008
-
-
-
-
-
-
-
-
2
-
-
8
-
2
-
-
-
-
-
-
-
-
8
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
8
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675484
Tanaka
Evolution of the cytoplasmic a ...
Arenicola brasiliensis
J. Mol. Evol.
65
616-625
2007
-
-
-
-
-
-
-
-
2
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661697
Uda
Origin and properties of cytop ...
Arenicola brasiliensis
FEBS J.
272
3521-3530
2005
-
-
1
-
-
-
-
-
2
-
2
2
-
5
-
-
1
-
-
1
5
-
7
-
5
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
2
2
-
-
-
1
-
2
5
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
661899
Uda
Phosphagen kinase of the giant ...
Arenicola brasiliensis, Riftia pachyptila
Int. J. Biol. Macromol.
37
54-60
2005
-
-
1
-
-
1
-
3
6
-
1
3
-
9
-
-
1
-
-
2
6
-
11
-
7
-
-
-
3
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
3
6
-
1
3
-
-
-
2
-
4
6
-
11
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
642485
Surholt
Taurocyamine kinase from body- ...
Arenicola marina
Eur. J. Biochem.
93
279-285
1979
-
-
-
-
-
3
-
4
2
1
2
-
-
2
-
-
1
-
-
2
1
2
1
1
-
1
1
-
-
2
2
1
-
-
1
-
-
-
-
-
-
-
3
-
-
-
4
2
1
2
-
-
-
-
1
-
2
1
2
1
1
1
1
-
-
2
2
1
1
-
-
-
-
-
-
642486
Kassab
ATP:taurocyamine and ATP:lombr ...
Arenicola marina
Biochim. Biophys. Acta
99
397-405
1965
-
-
-
-
-
-
4
-
-
-
1
-
-
1
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642487
van Thoai
Hypotaurocyamine phosphokinase ...
Arenicola marina
Biochim. Biophys. Acta
73
437-444
1963
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-