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Literature summary for 2.7.3.4 extracted from

  • Surholt, B.
    Taurocyamine kinase from body-wall musculature of the lugworm Arenicola marina (1979), Eur. J. Biochem., 93, 279-285.
    View publication on PubMed

General Stability

General Stability Organism
glycerol does not stabilize Arenicola marina
unstable to freezing Arenicola marina
Unstable to lyophilization Arenicola marina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
N-taurocyamine pH 8.0, 25°C Arenicola marina
0.83
-
N-phosphotaurocyamine pH 7.2, 25°C Arenicola marina
1.2
-
ADP pH 7.2, 25°C Arenicola marina
3.3
-
ATP pH 8.0, 25°C Arenicola marina

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Arenicola marina 5829
-
mitochondrion
-
Arenicola marina 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, maximal activity at 10 mM Arenicola marina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
gel filtration Arenicola marina
61000
-
ultracentrifugation Arenicola marina

Organism

Organism UniProt Comment Textmining
Arenicola marina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arenicola marina

Source Tissue

Source Tissue Comment Organism Textmining
body wall muscle
-
Arenicola marina
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1283
-
-
Arenicola marina

Storage Stability

Storage Stability Organism
-20°C, no decrease of activity when the crude extract is kept frozen for several months Arenicola marina
0°C, 0.1 M phosphate buffer, pH 7.2, saturated with mannitol, 0.02% NaN3, stable for more than 2 months Arenicola marina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + taurocyamine
-
Arenicola marina ADP + N-phosphotaurocyamine
-
r

Subunits

Subunits Comment Organism
More SDS-PAGE reveals 3 protein bands: 11000 Da, 13000-14000 Da and 21000-22000 Da Arenicola marina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
33
-
-
Arenicola marina

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 20°C: 70% of maximal activity, 45°C: less than 45% of maximal activity Arenicola marina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
synthesis of ATP Arenicola marina
8
-
synthesis of phosphotaurocyamine Arenicola marina

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8 pH 6.2: about 30% of maximal activity, pH 8.0: about 50% of maximal activity, synthesis of ATP Arenicola marina
7.2 8.7 pH 7.2: about 40% of maximal activity, pH 8.7: about 40% of maximal activity, synthesis of phosphotaurocyamine Arenicola marina

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 stable Arenicola marina

pI Value

Organism Comment pI Value Maximum pI Value
Arenicola marina separation of 8 bands between pH 6.2 and pH 7.8 with taurocyamine kinase activity, the highest activity appears at pH 7.3
-
additional information