Cloned (Comment) | Organism |
---|---|
gene Tb09.160.4560, sequence comparisons of Trypanosoma brucei isozymes AK1-3, recombinant expression of N-terminally His-tagged isozyme in Escherichia coli strain BL21(DE3) | Trypanosoma brucei brucei |
gene Tb927.9.6230, sequence comparisons of Trypanosoma brucei isozymes AK1-3 | Trypanosoma brucei brucei |
gene TbAK3, sequence comparisons of Trypanosoma brucei isozymes AK1-3 | Trypanosoma brucei brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of tetracycline-inducible PCF TbAK-RNAi cell line which allows the simultaneous silencing of all three TbAK isoforms. The TbAK-depleted cell line becomes about 400fold more sensitive to hydrogen peroxide | Trypanosoma brucei brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
ATP | pH 7.5, 28°C, recombinant isozyme AK1 | Trypanosoma brucei brucei | |
0.24 | - |
L-arginine | pH 7.5, 28°C, recombinant isozyme AK1 | Trypanosoma brucei brucei | |
0.32 | - |
ATP | pH 7.5, 37°C, recombinant isozyme AK1 | Trypanosoma brucei brucei | |
0.48 | - |
L-arginine | pH 7.5, 37°C, recombinant isozyme AK1 | Trypanosoma brucei brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isozyme TbAK3 is exclusively found in the cytosol. Isozyme TbAK3 lacks any apparent targeting sequences and is accordingly located in the cytosol of the parasite | Trypanosoma brucei brucei | 5829 | - |
flagellum | isozyme TbAK1 is exclusively found in the flagellum. The flagellar location of TbAK1 is dependent on a 22 amino acid long N-terminal sequence, which is sufficient for targeting a GFP-fusion protein to the trypanosome flagellum | Trypanosoma brucei brucei | - |
- |
glycosome | isozyme TbAK2 is exclusively found in the glycosome. The glycosomal location of TbAK2 is in agreement with the presence of a conserved peroxisomal targeting signal, the C-terminal tripeptide SNL | Trypanosoma brucei brucei | 20015 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Trypanosoma brucei brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
- |
Trypanosoma brucei brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | Trypanosoma brucei brucei | - |
ADP + Nomega-phospho-L-arginine | - |
r | |
ATP + L-arginine | Trypanosoma brucei brucei 927/4 | - |
ADP + Nomega-phospho-L-arginine | - |
r | |
ATP + L-arginine | Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 | - |
ADP + Nomega-phospho-L-arginine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | Q38EU9 | gene TbAK3 or Tb927.9.6290 | - |
Trypanosoma brucei brucei | Q38EV1 | gene Tb927.9.6230 | - |
Trypanosoma brucei brucei | Q38EV2 | - |
- |
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 | Q38EV2 | - |
- |
Trypanosoma brucei brucei 927/4 | Q38EU9 | gene TbAK3 or Tb927.9.6290 | - |
Trypanosoma brucei brucei 927/4 | Q38EV1 | gene Tb927.9.6230 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged isozyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography | Trypanosoma brucei brucei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | each TbAK isoform is differentially expressed in bloodstream and procyclic forms of Trypanosoma brucei, while the total cellular arginine kinase activity is 3fold higher in bloodstream form trypanosomes | Trypanosoma brucei brucei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | - |
Trypanosoma brucei brucei | ADP + Nomega-phospho-L-arginine | - |
r | |
ATP + L-arginine | - |
Trypanosoma brucei brucei 927/4 | ADP + Nomega-phospho-L-arginine | - |
r | |
ATP + L-arginine | - |
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 | ADP + Nomega-phospho-L-arginine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, recombinant His-tagged isozyme AK1, SDS-PAGE | Trypanosoma brucei brucei |
Synonyms | Comment | Organism |
---|---|---|
Tb09.160.4560 | - |
Trypanosoma brucei brucei |
TbAK2 | - |
Trypanosoma brucei brucei |
TbAK3 | - |
Trypanosoma brucei brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 37 | assay at | Trypanosoma brucei brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Trypanosoma brucei brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trypanosoma brucei brucei |
General Information | Comment | Organism |
---|---|---|
malfunction | elimination of the total cellular arginine kinase activity by RNA interference significantly decreases growth of procyclic form Trypanosoma brucei by 90% under standard culture conditions and is lethal for this life cycle stage in the presence of hydrogen peroxide | Trypanosoma brucei brucei |
physiological function | the phosphoarginine energy-buffering system of Trypanosoma brucei involves multiple arginine kinase isoforms with different subcellular locations. Increased arginine kinase activity improves growth of procyclic form Trypanosoma brucei during oxidative challenges with hydrogen peroxide | Trypanosoma brucei brucei |