Cloned (Comment) | Organism |
---|---|
recombinant expression of His6-tagged isozyme AK1 in Escherichia coli strain TB1 | Neocaridina denticulata |
recombinant expression of His6-tagged isozyme AK2 in Escherichia coli strain TB1 | Neocaridina denticulata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | determination of activation energy for transition state of AK reactions, thermodynamics | Neocaridina denticulata | |
0.131 | - |
L-arginine | pH 8.0, 15°C, recombinant enzyme | Neocaridina denticulata | |
0.165 | - |
L-arginine | pH 8.0, 20°C, recombinant enzyme | Neocaridina denticulata | |
0.166 | - |
L-arginine | pH 8.0, 15°C, recombinant enzyme | Neocaridina denticulata | |
0.179 | - |
L-arginine | pH 8.0, 17.5°C, recombinant enzyme | Neocaridina denticulata | |
0.196 | - |
L-arginine | pH 8.0, 17.5°C, recombinant enzyme | Neocaridina denticulata | |
0.278 | - |
ATP | pH 8.0, 25°C, recombinant enzyme | Neocaridina denticulata | |
0.29 | - |
L-arginine | pH 8.0, 22.5°C, recombinant enzyme | Neocaridina denticulata | |
0.302 | - |
L-arginine | pH 8.0, 20°C, recombinant enzyme | Neocaridina denticulata | |
0.337 | - |
ATP | pH 8.0, 15°C, recombinant enzyme | Neocaridina denticulata | |
0.339 | - |
ATP | pH 8.0, 17.5°C, recombinant enzyme | Neocaridina denticulata | |
0.34 | - |
ATP | pH 8.0, 17.5°C, recombinant enzyme | Neocaridina denticulata | |
0.344 | - |
L-arginine | pH 8.0, 25°C, recombinant enzyme | Neocaridina denticulata | |
0.376 | - |
ATP | pH 8.0, 25°C, recombinant enzyme | Neocaridina denticulata | |
0.399 | - |
ATP | pH 8.0, 20°C, recombinant enzyme | Neocaridina denticulata | |
0.405 | - |
ATP | pH 8.0, 22.5°C, recombinant enzyme | Neocaridina denticulata | |
0.422 | - |
ATP | pH 8.0, 15°C, recombinant enzyme | Neocaridina denticulata | |
0.439 | - |
L-arginine | pH 8.0, 25°C, recombinant enzyme | Neocaridina denticulata | |
0.454 | - |
ATP | pH 8.0, 30°C, recombinant enzyme | Neocaridina denticulata | |
0.456 | - |
L-arginine | pH 8.0, 30°C, recombinant enzyme | Neocaridina denticulata | |
0.465 | - |
ATP | pH 8.0, 20°C, recombinant enzyme | Neocaridina denticulata | |
0.538 | - |
L-arginine | pH 8.0, 30°C, recombinant enzyme | Neocaridina denticulata | |
0.779 | - |
L-arginine | pH 8.0, 35°C, recombinant enzyme | Neocaridina denticulata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Neocaridina denticulata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | Neocaridina denticulata | - |
ADP + Nomega-phospho-L-arginine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neocaridina denticulata | C0STY2 | isozyme AK1 | - |
Neocaridina denticulata | C0STY3 | isozyme AK2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged isozyme AK1 from Escherichia coli strain TB1 by nickel affinity chromatography | Neocaridina denticulata |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-arginine = ADP + Nomega-phospho-L-arginine | random-order, rapid-equilibrium kinetic mechanism | Neocaridina denticulata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | - |
Neocaridina denticulata | ADP + Nomega-phospho-L-arginine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AK1 | - |
Neocaridina denticulata |
AK2 | - |
Neocaridina denticulata |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 35 | activity profile, overview. The two isozymes AK1 and AK2 of Neocaridina denticulate show a marked difference in temperature dependence. The efficiency of AK2 decreases markedly with decreasing temperature, while that of AK1 does not decrease with decreasing temperature, suggesting that AK1 is a cold-adapted enzyme, attributable to the remarkable decrease inKiaARG with decreasing temperature, which enlarges its catalytic efficiency. AK1 is a psychrophilic enzyme | Neocaridina denticulata |
15 | 35 | activity profile, overview. The two isozymes AK1 and AK2 of Neocaridina denticulate show a marked difference in temperature dependence. The efficiency of AK2 decreases markedly with decreasing temperature, while that of AK1 does not decrease with decreasing temperature. AK2 is a mesophilic enzyme | Neocaridina denticulata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
108 | - |
L-arginine | pH 8.0, 15°C, recombinant enzyme | Neocaridina denticulata | |
134 | - |
L-arginine | pH 8.0, 17.5°C, recombinant enzyme | Neocaridina denticulata | |
170 | - |
L-arginine | pH 8.0, 15°C, recombinant enzyme | Neocaridina denticulata | |
174 | - |
L-arginine | pH 8.0, 20°C, recombinant enzyme | Neocaridina denticulata | |
183 | - |
L-arginine | pH 8.0, 17.5°C, recombinant enzyme | Neocaridina denticulata | |
196 | - |
L-arginine | pH 8.0, 22.5°C, recombinant enzyme | Neocaridina denticulata | |
217 | - |
L-arginine | pH 8.0, 25°C, recombinant enzyme | Neocaridina denticulata | |
244 | - |
L-arginine | pH 8.0, 20°C, recombinant enzyme | Neocaridina denticulata | |
272 | - |
L-arginine | pH 8.0, 25°C, recombinant enzyme | Neocaridina denticulata | |
343 | - |
L-arginine | pH 8.0, 30°C, recombinant enzyme | Neocaridina denticulata | |
459 | - |
L-arginine | pH 8.0, 35°C, recombinant enzyme | Neocaridina denticulata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Neocaridina denticulata |