Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.3.3 extracted from

  • Suzuki, T.; Kanou, Y.
    Two distinct arginine kinases in Neocaridina denticulate: psychrophilic and mesophilic enzymes (2014), Int. J. Biol. Macromol., 67, 433-438.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His6-tagged isozyme AK1 in Escherichia coli strain TB1 Neocaridina denticulata
recombinant expression of His6-tagged isozyme AK2 in Escherichia coli strain TB1 Neocaridina denticulata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information determination of activation energy for transition state of AK reactions, thermodynamics Neocaridina denticulata
0.131
-
L-arginine pH 8.0, 15°C, recombinant enzyme Neocaridina denticulata
0.165
-
L-arginine pH 8.0, 20°C, recombinant enzyme Neocaridina denticulata
0.166
-
L-arginine pH 8.0, 15°C, recombinant enzyme Neocaridina denticulata
0.179
-
L-arginine pH 8.0, 17.5°C, recombinant enzyme Neocaridina denticulata
0.196
-
L-arginine pH 8.0, 17.5°C, recombinant enzyme Neocaridina denticulata
0.278
-
ATP pH 8.0, 25°C, recombinant enzyme Neocaridina denticulata
0.29
-
L-arginine pH 8.0, 22.5°C, recombinant enzyme Neocaridina denticulata
0.302
-
L-arginine pH 8.0, 20°C, recombinant enzyme Neocaridina denticulata
0.337
-
ATP pH 8.0, 15°C, recombinant enzyme Neocaridina denticulata
0.339
-
ATP pH 8.0, 17.5°C, recombinant enzyme Neocaridina denticulata
0.34
-
ATP pH 8.0, 17.5°C, recombinant enzyme Neocaridina denticulata
0.344
-
L-arginine pH 8.0, 25°C, recombinant enzyme Neocaridina denticulata
0.376
-
ATP pH 8.0, 25°C, recombinant enzyme Neocaridina denticulata
0.399
-
ATP pH 8.0, 20°C, recombinant enzyme Neocaridina denticulata
0.405
-
ATP pH 8.0, 22.5°C, recombinant enzyme Neocaridina denticulata
0.422
-
ATP pH 8.0, 15°C, recombinant enzyme Neocaridina denticulata
0.439
-
L-arginine pH 8.0, 25°C, recombinant enzyme Neocaridina denticulata
0.454
-
ATP pH 8.0, 30°C, recombinant enzyme Neocaridina denticulata
0.456
-
L-arginine pH 8.0, 30°C, recombinant enzyme Neocaridina denticulata
0.465
-
ATP pH 8.0, 20°C, recombinant enzyme Neocaridina denticulata
0.538
-
L-arginine pH 8.0, 30°C, recombinant enzyme Neocaridina denticulata
0.779
-
L-arginine pH 8.0, 35°C, recombinant enzyme Neocaridina denticulata

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Neocaridina denticulata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-arginine Neocaridina denticulata
-
ADP + Nomega-phospho-L-arginine
-
r

Organism

Organism UniProt Comment Textmining
Neocaridina denticulata C0STY2 isozyme AK1
-
Neocaridina denticulata C0STY3 isozyme AK2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged isozyme AK1 from Escherichia coli strain TB1 by nickel affinity chromatography Neocaridina denticulata

Reaction

Reaction Comment Organism Reaction ID
ATP + L-arginine = ADP + Nomega-phospho-L-arginine random-order, rapid-equilibrium kinetic mechanism Neocaridina denticulata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine
-
Neocaridina denticulata ADP + Nomega-phospho-L-arginine
-
r

Synonyms

Synonyms Comment Organism
AK1
-
Neocaridina denticulata
AK2
-
Neocaridina denticulata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 35 activity profile, overview. The two isozymes AK1 and AK2 of Neocaridina denticulate show a marked difference in temperature dependence. The efficiency of AK2 decreases markedly with decreasing temperature, while that of AK1 does not decrease with decreasing temperature, suggesting that AK1 is a cold-adapted enzyme, attributable to the remarkable decrease inKiaARG with decreasing temperature, which enlarges its catalytic efficiency. AK1 is a psychrophilic enzyme Neocaridina denticulata
15 35 activity profile, overview. The two isozymes AK1 and AK2 of Neocaridina denticulate show a marked difference in temperature dependence. The efficiency of AK2 decreases markedly with decreasing temperature, while that of AK1 does not decrease with decreasing temperature. AK2 is a mesophilic enzyme Neocaridina denticulata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
108
-
L-arginine pH 8.0, 15°C, recombinant enzyme Neocaridina denticulata
134
-
L-arginine pH 8.0, 17.5°C, recombinant enzyme Neocaridina denticulata
170
-
L-arginine pH 8.0, 15°C, recombinant enzyme Neocaridina denticulata
174
-
L-arginine pH 8.0, 20°C, recombinant enzyme Neocaridina denticulata
183
-
L-arginine pH 8.0, 17.5°C, recombinant enzyme Neocaridina denticulata
196
-
L-arginine pH 8.0, 22.5°C, recombinant enzyme Neocaridina denticulata
217
-
L-arginine pH 8.0, 25°C, recombinant enzyme Neocaridina denticulata
244
-
L-arginine pH 8.0, 20°C, recombinant enzyme Neocaridina denticulata
272
-
L-arginine pH 8.0, 25°C, recombinant enzyme Neocaridina denticulata
343
-
L-arginine pH 8.0, 30°C, recombinant enzyme Neocaridina denticulata
459
-
L-arginine pH 8.0, 35°C, recombinant enzyme Neocaridina denticulata

Cofactor

Cofactor Comment Organism Structure
ATP
-
Neocaridina denticulata