Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) | Locusta migratoria manilensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | effects of the enzyme mutations on the enzyme's thermal inactivation andaggregation, the mutant enzymes show reduced thermal stability compared to the wild-type enzyme, overview | Locusta migratoria manilensis |
T273A | site-directed mutagenesis, the mutation leads to significant loss of activity, obviously decreased substrate synergism and structural stability compared to the wild-type enzyme, the enzyme structure is impaired and the enzyme protein in a partially unfolded state | Locusta migratoria manilensis |
T273D | site-directed mutagenesis, the mutation does not significantly affect the enzyme activity and structure | Locusta migratoria manilensis |
T273G | site-directed mutagenesis, the mutation leads to significant loss of activity, obviously decreased substrate synergism and structural stability compared to the wild-type enzyme, the enzyme structure is impaired and the enzyme protein in a partially unfolded state | Locusta migratoria manilensis |
T273S | site-directed mutagenesis, the mutation does not significantly affect the enzyme activity and structure | Locusta migratoria manilensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | dissociation consants of wild-type and mutant enzymes, overview | Locusta migratoria manilensis | |
0.94 | - |
L-arginine | pH 8.0, 30°C, wild-type enzyme | Locusta migratoria manilensis | |
0.95 | - |
L-arginine | pH 8.0, 30°C, recombinant wild-type enzyme | Locusta migratoria manilensis | |
0.98 | - |
L-arginine | pH 8.0, 30°C, recombinant mutant T273S | Locusta migratoria manilensis | |
1.04 | - |
L-arginine | pH 8.0, 30°C, recombinant mutant T273D | Locusta migratoria manilensis | |
1.27 | - |
ATP | pH 8.0, 30°C, recombinant wild-type enzyme | Locusta migratoria manilensis | |
1.28 | - |
ATP | pH 8.0, 30°C, recombinant mutant T273S | Locusta migratoria manilensis | |
1.29 | - |
ATP | pH 8.0, 30°C, wild-type enzyme | Locusta migratoria manilensis | |
1.32 | - |
ATP | pH 8.0, 30°C, recombinant mutant T273D | Locusta migratoria manilensis | |
1.64 | - |
ATP | pH 8.0, 30°C, recombinant mutant T273A | Locusta migratoria manilensis | |
1.7 | - |
ATP | pH 8.0, 30°C, recombinant mutant T273G | Locusta migratoria manilensis | |
3.05 | - |
L-arginine | pH 8.0, 30°C, recombinant mutant T273A | Locusta migratoria manilensis | |
3.18 | - |
L-arginine | pH 8.0, 30°C, recombinant mutant T273G | Locusta migratoria manilensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Locusta migratoria manilensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | Locusta migratoria manilensis | - |
ADP + Nomega-phospho-L-arginine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Locusta migratoria manilensis | A6M9J4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) | Locusta migratoria manilensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine | - |
Locusta migratoria manilensis | ADP + Nomega-phospho-L-arginine | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Locusta migratoria manilensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
54.16 | - |
ATP | pH 8.0, 30°C, recombinant mutant T273G | Locusta migratoria manilensis | |
54.16 | - |
L-arginine | pH 8.0, 30°C, recombinant mutant T273G | Locusta migratoria manilensis | |
60.97 | - |
ATP | pH 8.0, 30°C, recombinant mutant T273A | Locusta migratoria manilensis | |
60.97 | - |
L-arginine | pH 8.0, 30°C, recombinant mutant T273A | Locusta migratoria manilensis | |
143.2 | - |
ATP | pH 8.0, 30°C, recombinant mutant T273D | Locusta migratoria manilensis | |
143.2 | - |
L-arginine | pH 8.0, 30°C, recombinant mutant T273D | Locusta migratoria manilensis | |
151.3 | - |
ATP | pH 8.0, 30°C, recombinant mutant T273S | Locusta migratoria manilensis | |
151.3 | - |
L-arginine | pH 8.0, 30°C, recombinant mutant T273S | Locusta migratoria manilensis | |
159.4 | - |
ATP | pH 8.0, 30°C, recombinant wild-type enzyme | Locusta migratoria manilensis | |
159.4 | - |
L-arginine | pH 8.0, 30°C, recombinant wild-type enzyme | Locusta migratoria manilensis | |
163 | - |
ATP | pH 8.0, 30°C, wild-type enzyme | Locusta migratoria manilensis | |
163 | - |
L-arginine | pH 8.0, 30°C, wild-type enzyme | Locusta migratoria manilensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Locusta migratoria manilensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Locusta migratoria manilensis | |
ATP | - |
Locusta migratoria manilensis |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme structure homology modelling, overview | Locusta migratoria manilensis |
additional information | residue C271 is involved in the enzyme activity and constraining the orientation of the substrate arginine. Residue T273 interacts with C271 and plays a vital role in the enzyme activity, substrate synergism, and structural stability | Locusta migratoria manilensis |
physiological function | arginine kinase is a key enzyme for cellular energy metabolism, catalyzing the reversible phosphoyl transfer from phosphoarginine to ADP in invertebrates | Locusta migratoria manilensis |